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Production of Cellulosic Butyrate and 3-Hydroxybutyrate in Engineered Escherichia Coli Publisher Pubmed



Miscevic D1 ; Srirangan K2 ; Kefale T1 ; Abedi D1, 3 ; Mooyoung M1 ; Chou CP1
Authors
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Authors Affiliations
  1. 1. Department of Chemical Engineering, University of Waterloo, 200 University Avenue West, Waterloo, N2L 3G1, ON, Canada
  2. 2. Biotechnology Research Institute, National Research Council of Canada, Montreal, H4P 2R2, QC, Canada
  3. 3. Department of Drug & Food Control, Tehran University of Medical Sciences, Tehran, Iran

Source: Applied Microbiology and Biotechnology Published:2019


Abstract

Being the most abundant renewable organic substance on Earth, lignocellulosic biomass has acted as an attractive and cost-effective feedstock for biobased production of value-added products. However, lignocellulosic biomass should be properly treated for its effective utilization during biotransformation. The current work aimed to demonstrate biobased production of butyrate and 3-hydroxybutyrate (3-HB) in engineered Escherichia coli using pretreated and detoxified aspen tree (Populus tremuloides) wood chips as the feedstock. Various bioprocessing and genetic/metabolic factors limiting the production of cellulosic butyrate and 3-HB were identified. With these developed bioprocessing strategies and strain engineering approaches, major carbons in the hydrolysate, including glucose, xylose, and even acetate, could be completely dissimilated during shake-flask cultivation with up to 1.68 g L−1 butyrate, 8.95 g L−1 3-HB, and minimal side metabolites (i.e., acetate and ethanol) being obtained. Our results highlight the importance of consolidating bioprocess and genetic engineering strategies for effective biobased production from lignocellulosic biomass. © 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
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