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Genotoxicity of Noscapine Nanosuspension Prepared by Microfluidic Reactors on Hepg2 Cell Line Publisher



Azarian M1 ; Amani A2 ; Faramarzi MA3 ; Eidi A1 ; Divsalar A4
Authors
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Authors Affiliations
  1. 1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
  2. 2. Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
  3. 3. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

Source: Iranian Journal of Chemistry and Chemical Engineering Published:2020


Abstract

Noscapine is an antispasmodic alkaloid used as antitussive and anti-cough obtained from plants about Papaveraceae family which this benzylisoquinoline alkaloid and its synthetic subsidiaries (called noscapinoids) are being assessed for their anticancer potential. The present research aimed to investigate the induction of DNA destruction and viability of HepG2 tumor spheroid culture influenced by noscapine and nanosuspension of noscapine. Culture of HepG2 cells as spheroids was treated with different concentrations of noscapine for 24 h on Day 11. Afterward, viability assay and alkaline comet assay methods were applied to examine the viability and induced DNA destruction, respectively. Based on the results, no significant impact was observed from Tween 40 on the viability and DNA damage levels in comparison with the control (p > 0.05). Moreover, increasing noscapine concentration resulted in a dose-dependent reduction in viability of hepatic cancer cells and elevation of DNA damages, showing a correlation between rises of DNA damages and viability decline. © 2020, Iranian Institute of Research and Development in Chemical Industries. All rights reserved.
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