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The Evaluation of Wbp2nl-Related Genes Expression in Breast Cancer Publisher Pubmed



Nourashrafeddin S1, 5 ; Aarabi M2 ; Modarressi MH3 ; Rahmati M4 ; Nouri M1, 4
Authors
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Authors Affiliations
  1. 1. Women’s Reproductive Health Research Center, Tabriz University of Medical science, Tabriz, Iran
  2. 2. Department of Anatomy and Cell Biology, Queen’s University, Kingston, Canada
  3. 3. Department of Medical Genetics, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Biochemistry, Tabriz University of Medical science, Tabriz, Iran
  5. 5. Department of Obstetrics, Gynecology and Reproductive Sciences and Magee Womens Research Institute, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States

Source: Pathology and Oncology Research Published:2015


Abstract

Breast cancer is the most frequent cause of mortality in women all around the world; therefore, study on molecular aspects of breast cancer is necessary for finding new biomarkers. Recent studies have shown that WW Binding Protein 2 (WBP2) is an important protein for the oncogenic property of cancer. We have previously evaluated the WW Binding Protein 2 N-Terminal Like (WBP2NL) gene expression in cancerous cell line and breast tumor tissues, and reported changes in expression, which could increase tumorigenic cell growth. However, the molecular mechanisms of WBP2NL and its clinical relevance have not been investigated. In this study, the expression of WBP2NL-related genes in the invasive breast carcinoma and normal breast tissues was evaluated for the first time. Analysis of WBP2NL-related genes expression was performed with reverse transcription-PCR and real time-PCR detection method. The target genes studied were as follow: WW domain containing E3 ubiquitin protein ligase 1(WWP1), membrane associated guanylatekinase containing WW and PDZ domain-1 (MAGI1), neural precursor cell expressed developmentally down-regulated 4 (NEDD4), formin binding protein-4 (FNBP4), BCL2-associated athanogene-3 (BAG3), WW domain-containing oxidoreductase (WWOX), yes-associated protein-1 (YAP1), WW domain containing transcription regulator (WWTR1), member RAS oncogene family (RAB2A), and small G protein signaling modulator 3 (SGSM3). The expression of WWP1, BAG3, and WWTR1 was significantly increased in breast cancer. In contrast, the expression of WWOX, YAP1, RAB2A, and SGSM3 was significantly decreased. The MAGI1 and NEDD4 expression was increased, while the expression of FNBP4 was unchanged. These findings lead us to suggest that WBP2NL might play roles as an anti-apoptotic factor or co-activator to promote breast cancer cell survival and proliferation. © 2014, Aranyi Lajos Foundation.