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K-Casein Upregulates Osteogenic Differentiation on Bone Marrow Mesenchymal Stem Cells Cultured on Agarose Microcarriers Publisher



Tanimowo Aiyelabegan H1 ; Ebadi M1 ; Ali Kardar G2 ; Lotfibakhshaiesh N3 ; Abedin Dorkoosh F4 ; Ebrahimibarough S3 ; Sadroddiny E1
Authors
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Authors Affiliations
  1. 1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Immunology, Asthma and Allergy Research Institute, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: International Journal of Polymeric Materials and Polymeric Biomaterials Published:2020


Abstract

Microcarriers have been used as a three-dimensional substrates for cells cultivation and expansion. The present study aimed to fabricate a novel agarose-κ-casein microsphere, and assess its compatibility towards stem cells attachment, proliferation, survival, differentiation and osteogenic expression. Agarose microspheres were synthesized by emulsion method and activated with CNBr. Cells compatibility of the composite was observed at day 1, 4 and 7 by MTT, in addition FTIR, and SEM analysis were also performed. This microcarrier systems upregulated the expression of osteogenic differentiation markers on bone marrow mesenchymal stem cells cultured on the carrier systems (p < 0.0001). © 2019, © 2019 Taylor & Francis Group, LLC.