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Determination of Trace Amounts of Risperidone in Human Urine Sample by Hollow Fiber Liquid-Phase Microextraction Combined With High Performance Liquid Chromatography Publisher



Charmahali G1 ; Chaharmahali M2 ; Tafti FF3
Authors
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Authors Affiliations
  1. 1. Department of Medicinal Chemistry, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
  2. 2. Digestive Disease Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Chemistry, Faculty of Science, Islamic Azad University, Mashhad, Iran

Source: Biosciences Biotechnology Research Asia Published:2015


Abstract

Risperidone is an antipsychotic drug mainly used to treat schizophrenia. In this paper, for the first time a three-phase hollow uber liquid-phase microextraction (HF-LPME) method combined with high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection was presented to detection and quantification of risperidone in urine samples. Different factors affecting the HF-LPME extraction recovery were studied and optimized. Under optimal extraction condition, risperidone could be determined within the linearity range with a good correlation coefficient (r2 > 0.9980). The limit of detection for risperidone was 0.2 ng mL-1, and the intra- and inter-day relative standard deviations were no more than 1.2% and 3.2%, respectively. This procedure afforded a simple, sensitive and inexpensive method with a high extraction efficiency for determination of risperidone. Finally, it was applied to determination and quantification of risperidone in human urine samples.