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Species Identification of the Anopheles Fluviatilis Complex Using Phylogenetic Analysis Pcr-Sequencing in Southwestern Iran



Barghamadi Z1 ; Moosakazemi SH1 ; Oshaghi MA1 ; Vatandoost H1 ; Shahraki G2
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Authors Affiliations
  1. 1. Department of Medical Entomology and Vector Control, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Faculty of Health, Yasuj University of Medical Sciences, Yasuj, Iran

Source: Acta Medica Mediterranea Published:2016

Abstract

Introduction: Anopheles fluviatilis is one of the most important malaria vector in Iran. The aim of this study was to identify Anopheles fluviatilis complex species using proliferation and sequence analysis of ITS2 and also 28.S- D3parts of rDNA gene in southwestern Iran. Method: In this research Anopheles fluviatilis was caught from different areas of Kohgiluye and Boyer-Ahmad province at southwestern Iran at 1392. DNA was taken from 4 Anopheles fluviatilis selected samples and PCR tests of 28S- D3 part was done on sample's DNA and then after sequence of obtained results were identified and were compared with similar samples of Anopheles fluviatilis based on data's of gene word bank . Phylogenetic tree and individual sequences of samples were calculated. Results:PCR Phylogenetic analysis of r DNA 28S-D3 part of Anopheles fluviatilis confirmed that U genotype in Kohgiluye and Boyer-Ahmad province was identified. Gene length of r DNA 28S-D3 part between populations of this species was same (333bp). Conclusion: This study showed that Anopheles fluviatilis has species separate branch in southwestern Iran which is different with branch of southeastern Iran (Hormozgan, Kerman and Sistaan & Baluchestaan provinces).
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