Administration of 5-Hydroxydecanoate, a Selective Inhibitor of Mitochondrial Atp-Sensitive Potassium Channels, Inhibits Apelin-Induced Cardioprotection in Ischemia/Reperfusion Model of Male Rats



Chookalaei LG¹ ; Imani A¹ ; Faghihi M¹ ; Azizi Y² ; Rajani SF¹
Source: Indian Journal of Physiology and Pharmacology Published:2017

Abstract

Apelin, a recently discovered endogenous peptide has shown to protect myocardium against infarction in the animal model. The aim of this study was to evaluate the effects of mitochondrial ATP-sensitive potassium channels inhibition on myocardial protection afforded by apelin treatment during ischemia period. Thirty-two male Wistar rats were divided into four groups; (1) Ischemia/reperfusion, (2) Apelin + ischemia/reperfusion, (3) 5-hydroxydecanoate + Apelin + ischemia/reperfusion and (4) 5-hydroxydecanoate + ischemia/reperfusion. Hemodynamic parameters and infarct size were measured for all groups. There were no significant differences in hemodynamic functions during ischemia and reperfusion periods between the experimental groups. Further, there was a decrease in infarct size in apelin group when compared to ischemia/reperfusion group. However, selective inhibition of mitochondrial ATP-sensitive potassium channels by administration of 5-hydroxydecanoate, diminished the infarct-sparing effect of apelin. These findings suggest that apelin-induced protection was removed by using 5-hydroxydecanoate as a selective inhibitor of mitochondrial ATP-sensitive potassium channel. © 2017, Association of Physiologists and Pharmacologists of India. All rights reserved.