Real-Time Pcr Followed by High-Resolution Melting Analysis - a New Robust Approach to Evaluate Sccmec Typing of Methicillin-Resistant Staphylococcus Aureus

Real-Time Pcr Followed by High-Resolution Melting Analysis - a New Robust Approach to Evaluate Sccmec Typing of Methicillin-Resistant Staphylococcus Aureus Publisher Pubmed

Sadeghi Y¹ ; Salami SA² ; Kananizadeh P¹ ; Mozhgani SH³ ; Pourmand MR¹

Source: Future Microbiology Published:2019

Abstract

Aim: We designed a novel approach based on real-time PCR followed by high-resolution melting (HRM) analysis to determine the Staphylococcal cassette chromosome mec (SCCmec) types of methicillin-resistant Staphylococcus aureus strains, which we compared against the results of conventional multiplex PCR SCCmec typing. Methods: Multiplex PCR (for ccr and mec gene complexes) was carried out as conventional method. The HRM analysis was then designed using standard strains of each SCCmec type. Results: The M-PCR results included types III (33.33%), IV (43.33%) and V (23.33%). HRM analysis was able to distinguish all five types, which were used to set up the protocol with a sensitivity and specificity of 100% compared with the conventional method. Conclusion: This novel method for SCCmec typing has high specificity and sensitivity and can be conducted in a shorter period of time at lower costs. © 2019 2019 Future Medicine Ltd.

Style	Citing Format
MLA	Sadeghi Y, et al.. "Real-Time Pcr Followed by High-Resolution Melting Analysis - a New Robust Approach to Evaluate Sccmec Typing of Methicillin-Resistant Staphylococcus Aureus." Future Microbiology, vol. 14, no. 2, 2019, pp. 155-164.
APA	Sadeghi Y, Salami SA, Kananizadeh P, Mozhgani SH, Pourmand MR (2019). Real-Time Pcr Followed by High-Resolution Melting Analysis - a New Robust Approach to Evaluate Sccmec Typing of Methicillin-Resistant Staphylococcus Aureus. Future Microbiology, 14(2), 155-164.
Chicago	Sadeghi Y, Salami SA, Kananizadeh P, Mozhgani SH, Pourmand MR. "Real-Time Pcr Followed by High-Resolution Melting Analysis - a New Robust Approach to Evaluate Sccmec Typing of Methicillin-Resistant Staphylococcus Aureus." Future Microbiology 14, no. 2 (2019): 155-164.
Harvard	Sadeghi Y et al. (2019) 'Real-Time Pcr Followed by High-Resolution Melting Analysis - a New Robust Approach to Evaluate Sccmec Typing of Methicillin-Resistant Staphylococcus Aureus', Future Microbiology, 14(2), pp. 155-164.
Vancouver	Sadeghi Y, Salami SA, Kananizadeh P, Mozhgani SH, Pourmand MR. Real-Time Pcr Followed by High-Resolution Melting Analysis - a New Robust Approach to Evaluate Sccmec Typing of Methicillin-Resistant Staphylococcus Aureus. Future Microbiology. 2019;14(2):155-164.
BibTex	@article{ author = {Sadeghi Y and Salami SA and Kananizadeh P and Mozhgani SH and Pourmand MR}, title = {Real-Time Pcr Followed by High-Resolution Melting Analysis - a New Robust Approach to Evaluate Sccmec Typing of Methicillin-Resistant Staphylococcus Aureus}, journal = {Future Microbiology}, volume = {14}, number = {2}, pages = {155-164}, year = {2019} }
RIS	TY - JOUR AU - Sadeghi Y AU - Salami SA AU - Kananizadeh P AU - Mozhgani SH AU - Pourmand MR TI - Real-Time Pcr Followed by High-Resolution Melting Analysis - a New Robust Approach to Evaluate Sccmec Typing of Methicillin-Resistant Staphylococcus Aureus JO - Future Microbiology VL - 14 IS - 2 SP - 155 EP - 164 PY - 2019 ER -

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