Albumin Coated Copper-Cysteine Nanozyme for Reducing Oxidative Stress Induced During Sperm Cryopreservation

Albumin Coated Copper-Cysteine Nanozyme for Reducing Oxidative Stress Induced During Sperm Cryopreservation Publisher Pubmed

Dashtestani F¹ ; Ghourchian H¹ ; Najafi A²

Show Affiliations

1. Laboratory of Bioanalysis, Institute of Biochemistry & Biophysics, University of Tehran, P.O. Box 13145-1384, Tehran, Iran
2. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, P.O. Box 14155-6447, Tehran, Iran

Source: Bioorganic Chemistry Published:2018

Abstract

In the present work, SOD mimetic nanozyme (NACu-Cys) consisting of Cu-Cys complex and nano-albumin (NA) were synthesized. After characterizing the nanozyme, its superoxide dismutase (SOD) behavior was evaluated by inhibition of the pyrogallol autoxidation method. The results revealed that NACu-Cys exhibited SOD mimetic activity with a half inhibition concentration (IC 50 ) value of 7.0 × 10 − 3 µM and a turnover number (k cat ) of 5.4 × 10 7 s − 1 . In the next step, this nanozyme was applied as a protective agent against oxidative stress induced by sperm cryopreservation. Increasing the motility, raising the viability and reducing the apoptosis occurred as a result of NACu-Cys additions to human sperm freezing medium. Comparison between the natural SOD and SOD mimic behavior of NACu-Cys revealed that this nanoparticle has the ability to be used as oxidative stress decrescent during cryopreservation process. © 2018 Elsevier Inc.

Related Docs

1. Silver-Gold-Apoferritin Nanozyme for Suppressing Oxidative Stress During Cryopreservation, Materials Science and Engineering C (2019)

2. Aqueous Origanum Vulgare Extract Improves the Quality of Cryopreserved Human Spermatozoa Through Its Antioxidant Effects, Biopreservation and Biobanking (2020)

Experts (# of related papers)

Mahdi Vazirian (1)

Azim Hedayatpour (1)

Style	Citing Format
MLA	Dashtestani F, Ghourchian H, Najafi A. "Albumin Coated Copper-Cysteine Nanozyme for Reducing Oxidative Stress Induced During Sperm Cryopreservation." Bioorganic Chemistry, vol. 80, no. , 2018, pp. 621-630.
APA	Dashtestani F, Ghourchian H, Najafi A (2018). Albumin Coated Copper-Cysteine Nanozyme for Reducing Oxidative Stress Induced During Sperm Cryopreservation. Bioorganic Chemistry, 80(), 621-630.
Chicago	Dashtestani F, Ghourchian H, Najafi A. "Albumin Coated Copper-Cysteine Nanozyme for Reducing Oxidative Stress Induced During Sperm Cryopreservation." Bioorganic Chemistry 80, no. (2018): 621-630.
Harvard	Dashtestani F, Ghourchian H, Najafi A (2018) 'Albumin Coated Copper-Cysteine Nanozyme for Reducing Oxidative Stress Induced During Sperm Cryopreservation', Bioorganic Chemistry, 80(), pp. 621-630.
Vancouver	Dashtestani F, Ghourchian H, Najafi A. Albumin Coated Copper-Cysteine Nanozyme for Reducing Oxidative Stress Induced During Sperm Cryopreservation. Bioorganic Chemistry. 2018;80():621-630.
BibTex	@article{ author = {Dashtestani F and Ghourchian H and Najafi A}, title = {Albumin Coated Copper-Cysteine Nanozyme for Reducing Oxidative Stress Induced During Sperm Cryopreservation}, journal = {Bioorganic Chemistry}, volume = {80}, number = {}, pages = {621-630}, year = {2018} }
RIS	TY - JOUR AU - Dashtestani F AU - Ghourchian H AU - Najafi A TI - Albumin Coated Copper-Cysteine Nanozyme for Reducing Oxidative Stress Induced During Sperm Cryopreservation JO - Bioorganic Chemistry VL - 80 IS - SP - 621 EP - 630 PY - 2018 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract