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Cpg Islands Methylation Analysis of Cdh11, Epha5, and Hs3st2 Genes in Gastric Adenocarcinoma Patients Publisher Pubmed



Eyvazi S1, 2 ; Khamaneh AM3 ; Tarhriz V4 ; Bandehpour M2 ; Hejazi MS4 ; Sadat ATE5 ; Sepehri B6
Authors
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Authors Affiliations
  1. 1. Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  2. 2. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Department of Molecular Medicine, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
  4. 4. Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  5. 5. Pathology Department, Imam Reza Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
  6. 6. Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Source: Journal of Gastrointestinal Cancer Published:2020


Abstract

Purpose: Gastric cancer is an aggressive disease which is the fourth prevalent malignancy in the world. Beside the genetic factors, epigenetic alterations such as promoter CpG island hyper methylation are involved in the emergence of gastric cancer. Herein, we investigated the methylation status of CDH11, EphA5, and HS3ST2 genes in patients with and without gastric adenocarcinoma for the first time. Methods: In the study 40 paraffin-embedded tissue sections from gastric adenocarcinoma patients and 40 specimens from patients with functional dyspepsia were taken. DNA extraction was performed using a modified salting out method. Epizen DNA methylation kit was used to the bisulfite DNA conversion. The methylation status of CDH11, EphA5, and HS3ST2 genes were analyzed by methylation-specific PCR (MSP) technique. Results: Among the 80 specimens, 71 DNA samples were achieved (34 gastric adenocarcinoma patients and 37 control patients). The results showed that CDH11, EphA5, and HS3ST2 genes are methylated in 28 (82.45%), 19 (55.88%), and 26 (76.47%) of 34 DNA samples from gastric adenocarcinoma patients, respectively, whereas, these genes are methylated in 7 (18.91%), 9 (24.32%) and 7 (18.91%) of 37 samples from noncancerous patients, respectively. Statistical analyses using a chi-squared test showed that there is a statistically significant difference in methylation level of CDH11, EphA5, and HS3ST2 genes between gastric cancer and uncancerous patients (p < 0.05). Conclusion: To the best of our knowledge, this is the first report on methylation of CDH11, EphA5, and HS3ST2 promoters’ in gastric adenocarcinoma patients using MSP. Identification of novel cancer-related molecular mechanisms can be useful in detection of new treatment strategies. © 2019, Springer Science+Business Media, LLC, part of Springer Nature.
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