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New Thioxothiazolidinyl-Acetamides Derivatives As Potent Urease Inhibitors: Design, Synthesis, in Vitro Inhibition, and Molecular Dynamic Simulation Publisher Pubmed



Dastyafteh N1 ; Noori M1 ; Nazari Montazer M2 ; Zomorodian K3 ; Yazdanpanah S3 ; Iraji A4, 5 ; Khalili Ghomi M1 ; Javanshir S6 ; Asadi M2 ; Dianatpour M3 ; Biglar M7 ; Larijani B1 ; Amanlou M2, 7 ; Mahdavi M1
Authors
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Authors Affiliations
  1. 1. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medical Mycology and Parasitology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  4. 4. Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  5. 5. Central Research Laboratory, Shiraz University of Medical Sciences, Shiraz, Iran
  6. 6. Department of Chemistry, Iran University of Science and Technology, Tehran, Iran
  7. 7. Drug Design and Development Research Center, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran

Source: Scientific Reports Published:2023


Abstract

To identify potent urease inhibitors, in the current study, a series of thioxothiazolidinyl-acetamides were designed and synthesized. The prepared compounds were characterized by spectroscopic techniques, including FTIR, 1HNMR, 13CNMR, and elemental analysis. In the enzymatic assessments, it was demonstrated that all derivatives had significant urease inhibition with IC50 values in the range of 1.473–9.274 µM in comparison with the positive control hydroxyurea (IC50 = 100.21 ± 2.5 µM) and thiourea (IC50 = 23.62 ± 0.84 µM). Compound 6i (N-benzyl-3-butyl-4-oxo-2-thioxothiazolidine-5-carboxamide) was the most active agent with an IC50 value of 1.473 µM. Additionally, kinetic investigation and in silico assessments of 6i was carried out to understand the type of inhibition and behavior of the most potent derivative within the binding site of the enzyme. Noteworthy, the anti-urease assay against P. vulgaris revealed 6e and 6i as the most active agents with IC50 values of 15.27 ± 2.40 and 17.78 ± 3.75 µg/mL, respectively. Antimicrobial evaluations of all compounds reveal that compounds 6n and 6o were the most potent antimicrobial agents against the standard and resistant S. aureus. 6n and 6o also showed 37 and 27% inhibition in the development of biofilm by S. aureus at 512 µg/ml. Furthermore, the MTT test showed no toxicity up to 100 µM. Taken together, the study suggests that the synthesized thioxothiazolidinyl-acetamides bases derivatives may serve as potential hits as urease inhibitors. © 2023, The Author(s).
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