Effect of Metformin on Germ Cell-Specific Apoptosis, Oxidative Stress and Epididymal Sperm Quality After Testicular Torsion/Detorsion in Rats

Effect of Metformin on Germ Cell-Specific Apoptosis, Oxidative Stress and Epididymal Sperm Quality After Testicular Torsion/Detorsion in Rats Publisher Pubmed

Ghasemnejadberenji M^{1, 2, 3} ; Ghazikhansari M¹ ; Yazdani I^{1, 2} ; Nobakht M⁴ ; Abdollahi A⁵ ; Ghasemnejadberenji H⁶ ; Mohajer Ansari J⁴ ; Pashapour S⁷ ; Dehpour AR^{1, 2}

Show Affiliations

1. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Pharmacology, Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran
4. Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
5. Department of Pathology, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran
6. Department of Anatomy and Reproductive Biology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7. Department of Pathology, Urmia University of Medical Science, Urmia, Iran

Source: Andrologia Published:2018

Abstract

Summary: The study was designed to evaluate the effects of metformin on apoptosis and epididymal sperm quality in a rat testicular ischaemia/reperfusion (I/R) injury model. A total of 72 male rats were divided into four groups (n = 18 for each group): group 1 (sham-operated group), group 2 (metformin group), group 3 (torsion/detorsion [T/D] + saline) and group 4 (T/D + 300 mg kg−1 metformin). Testicular torsion was achieved by rotating the right testis 720° in a clockwise direction for 1 hr. Tissue malondialdehyde (MDA) level and caspase-3 activity increased and the activities of catalase, superoxide dismutase and glutathione peroxidase decreased in comparison with sham-operated group 4 hr after detorsion (p <.001). In six rats of each group 24 hr after detorsion, histopathological changes and germ cell apoptosis were significantly deteriorated by measuring mean of seminiferous tubule diameters (MSTD) and TUNEL test. Moreover, 30 days after T/D, sperm concentration and motility were examined in six animals per group. Metformin pre-treatment reduced MDA and caspase-3 levels and normalised antioxidant enzyme activities 4 hr after detorsion, and germ cell apoptosis was significantly decreased, and the MSTD, as well as sperm functions, was significantly improved. Reduction in oxidative stress and apoptosis may have a major role in cytoprotective effects of metformin. © 2017 Blackwell Verlag GmbH

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Style	Citing Format
MLA	Ghasemnejadberenji M, et al.. "Effect of Metformin on Germ Cell-Specific Apoptosis, Oxidative Stress and Epididymal Sperm Quality After Testicular Torsion/Detorsion in Rats." Andrologia, vol. 50, no. 2, 2018, pp. -.
APA	Ghasemnejadberenji M, Ghazikhansari M, Yazdani I, Nobakht M, Abdollahi A, Ghasemnejadberenji H, Mohajer Ansari J, Pashapour S, Dehpour AR (2018). Effect of Metformin on Germ Cell-Specific Apoptosis, Oxidative Stress and Epididymal Sperm Quality After Testicular Torsion/Detorsion in Rats. Andrologia, 50(2), -.
Chicago	Ghasemnejadberenji M, Ghazikhansari M, Yazdani I, Nobakht M, Abdollahi A, Ghasemnejadberenji H, Mohajer Ansari J, Pashapour S, Dehpour AR. "Effect of Metformin on Germ Cell-Specific Apoptosis, Oxidative Stress and Epididymal Sperm Quality After Testicular Torsion/Detorsion in Rats." Andrologia 50, no. 2 (2018): -.
Harvard	Ghasemnejadberenji M et al. (2018) 'Effect of Metformin on Germ Cell-Specific Apoptosis, Oxidative Stress and Epididymal Sperm Quality After Testicular Torsion/Detorsion in Rats', Andrologia, 50(2), pp. -.
Vancouver	Ghasemnejadberenji M, Ghazikhansari M, Yazdani I, Nobakht M, Abdollahi A, Ghasemnejadberenji H, et al.. Effect of Metformin on Germ Cell-Specific Apoptosis, Oxidative Stress and Epididymal Sperm Quality After Testicular Torsion/Detorsion in Rats. Andrologia. 2018;50(2):-.
BibTex	@article{ author = {Ghasemnejadberenji M and Ghazikhansari M and Yazdani I and Nobakht M and Abdollahi A and Ghasemnejadberenji H and Mohajer Ansari J and Pashapour S and Dehpour AR}, title = {Effect of Metformin on Germ Cell-Specific Apoptosis, Oxidative Stress and Epididymal Sperm Quality After Testicular Torsion/Detorsion in Rats}, journal = {Andrologia}, volume = {50}, number = {2}, pages = {-}, year = {2018} }
RIS	TY - JOUR AU - Ghasemnejadberenji M AU - Ghazikhansari M AU - Yazdani I AU - Nobakht M AU - Abdollahi A AU - Ghasemnejadberenji H AU - Mohajer Ansari J AU - Pashapour S AU - Dehpour AR TI - Effect of Metformin on Germ Cell-Specific Apoptosis, Oxidative Stress and Epididymal Sperm Quality After Testicular Torsion/Detorsion in Rats JO - Andrologia VL - 50 IS - 2 SP - EP - PY - 2018 ER -

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