Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Analysis of Sdc2 Gene Promoter Methylation in Whole Blood for Noninvasive Early Detection of Colorectal Cancer Publisher Pubmed



Siri G1 ; Alesaeidi S2 ; Dizghandi SE3 ; Alani B4 ; Mosallaei M5, 6 ; Soosanabadi M3, 7
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Internal Medicine, Tehran University of Medical Sciences, Amiralam Hospital, Tehran, Iran
  2. 2. Department of Rheumatology and Internal Medicine, Rheumatology Research Center, Tehran University of Medical Sciences, Amir-Alam Hospital, Tehran, Iran
  3. 3. Department of Medical Genetics, Semnan University of Medical Sciences, Semnan, Iran
  4. 4. Department of Applied Cell Sciences, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran
  5. 5. School of Medicine, Aja University of Medical Science, Tehran, Iran
  6. 6. Department of Medical Genetics, Isfahan University of Medical Sciences, Isfahan, Iran
  7. 7. Abnormal Uterine Bleeding Research Center, Semnan University of Medical Sciences, Semnan, Iran

Source: Journal of Cancer Research and Therapeutics Published:2022


Abstract

Objectives: Considering the limitations of the current approaches to colorectal cancer (CRC) screening, scientists strived to find noninvasive and more powerful biomarkers for the early diagnosis of CRC.Nowadays, there are different sources of biomarkers for CRC diagnosis.Blood-based samples including circulating cell-free tumor DNA (ctDNA) and DNA extracted from leukocytes in peripheral blood might be promising sources of noninvasive cancer biomarkers such as cancer-specific methylation patterns.In this study, we aimed to evaluate the noninvasive early diagnosis of CRC via quantitative promotor methylation analysis of SDC2 gene in whole blood.Materials and Methods: Sixty-five CRC patients and 65 healthy participants were enrolled to assess promoter methylation of SDC2 gene in whole blood using the methylation quantification endonuclease-resistant DNA (MethyQESD) technique.Results: Our findings demonstrated drastic hypermethylation of SDC2 in blood samples from CRC subjects (37.91%) compared with non-malignant individuals (17.02%) (P < 0.001).The sensitivity for detection of CRC by methylation of SDC2 was 81.54%, with a specificity of 69.23%.The ROC curve analysis demonstrated that the AUC was 0.847 (P < 0.001), indicating that the status of SDC2 promoter methylation in whole blood is an excellent biomarker of CRC diagnosis.Furthermore, our results showed that methylation level in CRC patients significantly increased in higher tumor stages, demonstrating that an increased percentage of methylation is correlated with tumor progression (P < 0.001).Conclusion: SDC2 promoter methylation status in blood samples is a valuable cancer biomarker and holds high power and accuracy in distinguishing CRC patients from healthy subjects in the early stages of the disease. © 2021 Journal of Cancer Research and Therapeutics.
Related Docs
View other Related Docs
1. Hypermethylation of Mgmt Gene Promoter in Peripheral Blood Mononuclear Cells As a Noninvasive Biomarker for Colorectal Cancer Diagnosis, Advanced Biomedical Research (2023)
2. Tusc3 Methylation in Peripheral Blood Cells As a Biomarker for Diagnosis of Colorectal Cancer, Advanced Biomedical Research (2023)
3. Pattern of Participation in Colorectal Cancer Screening From a Population-Based Screening Program in Iran, Archives of Iranian Medicine (2024)
4. Methylation of Fbn1, Spg20, Itf2, Runx3, Snca, Mlh1, and Sept9 Genes in Circulating Cell-Free Dna As Biomarkers of Colorectal Cancer, Cancer Biomarkers (2022)
5. Naringenin: A Potential Flavonoid Phytochemical for Cancer Therapy, Life Sciences (2022)
6. Advances in Blood Dna Methylation-Based Assay for Colorectal Cancer Early Detection: A Systematic Updated Review, Gastroenterology and Hepatology from Bed to Bench (2024)
7. Opium Use and Risk of Colorectal Cancer: A Multi-Center Case-Referent Study in Iran, Acta Oncologica (2023)
8. Deciphering the Oncogenic Landscape: Unveiling the Molecular Machinery and Clinical Significance of Lncrna Tmpo-As1 in Human Cancers, Pathology Research and Practice (2024)
Experts (# of related papers)
View all Related Experts
Reza Malekzadeh (2)
Goli Siri (2)
Other Related Docs
9. Alterations of the Gut Microbiome and Metabolome Following: A Opportunity for Early Detection of Colorectal Cancer, Current Cancer Therapy Reviews (2025)
10. The Association Between Vitamin C and Breast Cancer, Prostate Cancer and Colorectal Cancer: A Systematic Review and Meta-Analysis, Clinical Nutrition ESPEN (2025)
11. Demethylation of Cdkn2a in Systemic Lupus Erythematosus and Rheumatoid Arthritis: A Blood Biomarker for Diagnosis and Assessment of Disease Activity, Clinical Rheumatology (2023)
12. 16S Rrna Sequencing Analysis of the Oral and Fecal Microbiota in Colorectal Cancer Positives Versus Colorectal Cancer Negatives in Iranian Population, Gut Pathogens (2024)
13. Toxic Mechanisms of Cadmium and Exposure As a Risk Factor for Oral and Gastrointestinal Carcinomas, Human and Experimental Toxicology (2023)
14. The Importance of Evaluating Serum Levels of Tumor Markers M2-Pk and Inhibin a in Patients Undergoing Colonoscopy, Technology in Cancer Research and Treatment (2023)
15. The Association Between Dietary Folate Intake and Risk of Colorectal Cancer Incidence: A Systematic Review and Dose‒Response Meta-Analysis of Cohort Studies, Heliyon (2024)
16. The Therapeutic Effect of Exosomes From Mesenchymal Stem Cells on Colorectal Cancer: Toward Cell-Free Therapy, Pathology Research and Practice (2022)
17. Significant Heightened Methylation Levels of Runx3 Gene Promoter in Patients With Systemic Lupus Erythematosus, Lupus (2024)
18. The Burden of Colorectal Cancer in Yazd Province in 2016, Iranian Journal of Epidemiology (2022)
19. Heterogeneous Expression of Long Noncoding Rna Rp11-109D20.2: Insights Into Regulatory Gene Expression Roles in Colon Cancer, Iranian Journal of Basic Medical Sciences (2025)
20. Colorectal Cancer: Epidemiology, Risk Factors, and Prevention, Cancers (2024)
21. Mortality Rates and Years of Life Lost Due to Cancer in Iran: Analysis of Data From the National Death Registration System, 2016, International Journal of Cancer Management (2022)
22. Incidence and Predicting Factors of Inadequate Bowel Preparation for Colonoscopy: A Cross-Sectional Study, JGH Open (2024)
23. Naturally Nutrient Rich (Nnr) Score and the Risk of Colorectal Cancer: A Case-Control Study, BMJ Open Gastroenterology (2023)
24. Association Between Probiotics and Modulation of Gut Microbial Community Composition in Colorectal Cancer Animal Models: A Systematic Review (2010-2021), Canadian Journal of Infectious Diseases and Medical Microbiology (2023)