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Network Analysis of 20S-Ginsenoside Rg3 Effect on Human Colorectal Adenocarcinoma Cell Line Ht-29 Publisher



Azodi MZ1 ; Arjmand B2 ; Tavirani MR3 ; Khodadoost M4 ; Nejad MR5 ; Ahmadi N4 ; Okhovatian F6
Authors
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Authors Affiliations
  1. 1. Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Research Institute for Gastroenterology and Liver Diseases, Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  6. 6. Physiotherapy Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Research Journal of Pharmacognosy Published:2022


Abstract

Background and objectives: 20S-Ginsenoside Rg3 is a pharmacological active compound of ginseng. Evidences indicate that S20-Rg3 as an anti-cancer factor plays role in prevention and treatment of cancer. In the present study, proteomic data of 20S-ginsenoside Rg3 effect on human colorectal adenocarcinoma cell line HT-29 was analyzed via network analysis to understand more details about the molecular events. Methods: The differentially expressed proteins (DEPs) related to the effect of 20S-ginsenoside Rg3 on human colorectal adenocarcinoma cell line HT-29 were extracted from literature and analyzed via protein-protein interaction (PPI) network. The central nodes of the network were determined based on degree value and betweenness centrality. Results: Eight DEPS plus 100 added first neighbors were included in the PPI network. Five central nodes as hub-bottlenecks including ACTB, GAPDH, TP53, AKT1, and ALB among the added first neighbors and ANXA5 as hub-bottleneck and GSTP1 and PCNA as bottlenecks among the queried DEPs were introduced. Conclusion: PCNA, GSTP1, and ANxA5 as cell protective proteins are the crucial targeted proteins by 20S-ginsenoside Rg3 in the treated cell line HT-29. Up-regulation of GSTP1 and ANXA5 is correspondent to the cell protective property of 20S-ginsenoside Rg3, and down-regulation of PCNA refers to the opposite effect. It seems that cell protective roles of 20S-ginsenoside Rg3 are accompanied with the possible side effects. © 2022. Open access.
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