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Comparative Restoration of Acute Liver Failure by Menstrual Blood Stem Cells Compared With Bone Marrow Stem Cells in Mice Model Publisher Pubmed



Fathikazerooni M1 ; Tavoosidana G1 ; Taghizadehjahed M2 ; Khanjani S2 ; Golshahi H3 ; Gargett CE4 ; Edalatkhah H2 ; Kazemnejad S2
Authors
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Authors Affiliations
  1. 1. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
  3. 3. Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  4. 4. The Ritchie Centre, Hudson Institute of Medical Research, Department of Obstetrics and Gynaecology, Monash University, Melbourne, Victoria, Australia

Source: Cytotherapy Published:2017


Abstract

Background aims The application of menstrual blood stem cells (MenSCs) in regenerative medicine is gaining increasing attention. The aim of this study was to investigate the therapeutic potential of MenSCs compared with bone marrow–derived stem cells (BMSCs) in an animal model of CCl4-induced acute hepatic failure. Methods Injured Balb/C mice were divided into multiple groups and received MenSCs, BMSCs or hepatocyte progenitor-like (HPL) cells derived from these cells. Results Tracking of green fluorescent protein–labeled cells showed homing of cells in injured areas of the liver. In addition, the liver engraftment of MenSCs was shown by immunofluorescence staining using anti-human mitochondrial antibody. Microscopically examination, periodic acid-Schiff and Masson's trichrome staining of liver sections demonstrated the considerable liver regeneration post–cell therapy in all groups. Assessment of serum parameters including aspartate aminotransferase, alanine aminotransferase, total bilirubin, urea and cholesterol at day 7 exhibited significant reduction, such that this downward trend continued significantly until day 30. The restoration of liver biochemical markers, changes in mRNA levels of hepatic markers and the suppression of inflammatory markers were more significant in the MenSC-treated group compared with the BMSC-treated group. On the other hand, HPL cells in reference to undifferentiated cells had better effectiveness in the treatment of the acute liver injury. Conclusions Our data show that MenSCs may be considered an appropriate alternative stem cell population to BMSCs for treatment of acute liver failure. © 2017 International Society for Cellular Therapy
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