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Comparison of Spag11a Gene Expression in Infertile Men With Grade 1 and 2 Varicocele Before and After Treatment Publisher Pubmed



Amiri S1, 2 ; Gholizadeh L3 ; Rasti A4, 5 ; Peymani M2 ; Mirjalili SAM6 ; Vahidi SA6 ; Kalantar SM4
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Authors Affiliations
  1. 1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  2. 2. Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
  3. 3. Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  4. 4. Abortion Research Centre, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Science, Yazd, Iran
  5. 5. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Andorology, Yazd Reproduction Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Source: Jornal Brasileiro de Reproducao Assistida Published:2024


Abstract

Objective: Sperm Associated Antigen 11A (SPAG11A) protein is a family of the epididymis-specific secretory proteins implicated in sperm maturation and function. Varicocele might cause pathophysiological difficulties in the testis and epididymis, with a harmful effect on the environment for spermatogenesis and sperm maturation. The aim of this study was to evaluate the expression level of the SPAG11A gene and sperm parameters in infertile men with grade 1 and 2 varicocele before and after treatment. Methods: Semen specimens were collected from 20 infertile men with varicocele pre-and post-treatment and 10 healthy volunteers. Semen analysis was conducted according to world health organization guidelines. Real time PCR (qRT-PCR) reaction was applied for determination of SPAG11A mRNA expression. Results: The results showed that there was a significant difference between the concentration and normal morphology between pre-and post-treatment groups and the controls. There were significant differences between pre-treatment and control groups in terms of progressive and non-progressive mobility. SPAG11A mRNA levels were significantly lower in the pre-treatment group than in healthy control subjects (p=0.007). There was no statistically significant difference in the expression of SPAG11A as well as semen parameters in the posttreatment group compared to the pre-treatment group. Conclusions: SPAG11A gene expression and semen parameters may be affected by varicocele. Whether varicocele treatment is an effective approach to reduce the adverse effect of this disease on SPAG11A expression and semen parameters needs further investigation. © 2024, SBRA - Associacao Brasileira de Reproducao Assistida (Brazilian Society of Assisted Reproduction). All rights reserved.
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2. Consensus and Diversity in the Management of Varicocele for Male Infertility: Results of a Global Practice Survey and Comparison With Guidelines and Recommendations, World Journal of Men's Health (2022)
3. Can Melissa Officinalis Improve Chromatin Structure and Sperm Parameters in a Rat Model of Varicocele?, Andrologia (2018)
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