Β-Propiolactone, Its Application and Assay Methods

Β-Propiolactone, Its Application and Assay Methods Publisher Pubmed

Mirjalili SZ ; Sabourian R ; Chavoshi F ; Shirangi M ; Tamiji Z ; Kobarfard F ; Amini M

Source: Analytical Methods Published:2025

Abstract

Vaccination is a crucial tool in the prevention of infectious diseases and has saved countless lives worldwide. One of the most commonly used techniques for producing vaccines is viral deactivation. β-Propiolactone (BPL) is an agent used to inactivate and sterilize biological products, including vaccines. BPL is favored for its ability to preserve virus capsid proteins and maintain high immunogenicity. However, BPL is an alkylating agent with potential carcinogenic properties, making it unacceptable for residual amounts to be present in the final vaccine product. Therefore, it is necessary to eliminate any trace amounts of BPL remaining after the inactivation process to ensure vaccine safety. This review aims to discuss different methods of viral inactivation, with a specific focus on BPL, and emphasize the necessity of accurately measuring its presence. The advantages and limitations of various analytical techniques that have proven effective in quantifying BPL, with a primary focus on gas chromatography (GC) and high-performance liquid chromatography (HPLC), are compared. These methods discussed in this paper offer valuable insights for quality control in vaccine production, ensuring product safety for patients. © 2025 Elsevier B.V., All rights reserved.

Style	Citing Format
MLA	Mirjalili SZ, et al.. "Β-Propiolactone, Its Application and Assay Methods." Analytical Methods, vol. 17, no. 38, 2025, pp. 7608-7617.
APA	Mirjalili SZ, Sabourian R, Chavoshi F, Shirangi M, Tamiji Z, Kobarfard F, Amini M (2025). Β-Propiolactone, Its Application and Assay Methods. Analytical Methods, 17(38), 7608-7617.
Chicago	Mirjalili SZ, Sabourian R, Chavoshi F, Shirangi M, Tamiji Z, Kobarfard F, Amini M. "Β-Propiolactone, Its Application and Assay Methods." Analytical Methods 17, no. 38 (2025): 7608-7617.
Harvard	Mirjalili SZ et al. (2025) 'Β-Propiolactone, Its Application and Assay Methods', Analytical Methods, 17(38), pp. 7608-7617.
Vancouver	Mirjalili SZ, Sabourian R, Chavoshi F, Shirangi M, Tamiji Z, Kobarfard F, et al.. Β-Propiolactone, Its Application and Assay Methods. Analytical Methods. 2025;17(38):7608-7617.
BibTex	@article{ author = {Mirjalili SZ and Sabourian R and Chavoshi F and Shirangi M and Tamiji Z and Kobarfard F and Amini M}, title = {Β-Propiolactone, Its Application and Assay Methods}, journal = {Analytical Methods}, volume = {17}, number = {38}, pages = {7608-7617}, year = {2025} }
RIS	TY - JOUR AU - Mirjalili SZ AU - Sabourian R AU - Chavoshi F AU - Shirangi M AU - Tamiji Z AU - Kobarfard F AU - Amini M TI - Β-Propiolactone, Its Application and Assay Methods JO - Analytical Methods VL - 17 IS - 38 SP - 7608 EP - 7617 PY - 2025 ER -

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