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Conjugated Linoleic Acid Stimulates Apoptosis in Rh and Tehran Strains of Toxoplasma Gondii, in Vitro



Shamseddin J1 ; Akhlaghi L1 ; Razmjou E1 ; Shojaee S2 ; Monavari SHR3 ; Tajik N4 ; Ebrahimi SA5 ; Meamar AR1
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Authors Affiliations
  1. 1. Dept. Of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Dept. Of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Dept. Of Virology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Dept. Of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  5. 5. Dept. Of Pharmacology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Parasitology Published:2015

Abstract

Background: The aim of this study was to evaluate the effects of conjugated linoleic acid (CLA) on apoptosis of tachyzoites of T. gondii, RH strain (type I) and the cyst-forming Tehran strain (type II) in vitro. Methods: Toxoplasma strains were injected into the peritoneal cavity of BALB/c mice. The Tehran strain forms cysts in the brain of mice. Bradyzoites within the cysts are reactivated to proliferative tachyzoites, by dexamethasone. Tachyzoites were aspirated from the peritoneum of infected mice, and the percentage of viable parasites was estimated with trypan blue staining. Tachyzoites were inoculated into HeLa cells cultivated in DMEM medium. Different concentrations of CLA were evaluated on T. gondii in HeLa cells by the tetrazolium (MTT) colorimetric assay. Differentiation between apoptosis and cell death was determined by flow cytometry using Annexin V and propidium iodide (PI) double staining. The statistical analysis performed by GraphPad Prism version 6.00. Results: CLA induces apoptosis in virulent (RH) and avirulent (Tehran) strains of T. gondii. The results of MTT indicated that CLA could decrease the proliferation of tachyzoites of both strains in HeLa cells. Conclusion: Conjugated linoleic acid has anti-toxoplasmacidal activity on tachyzoites of T. gondii. Therefore, we recommended further studies on this component in order to achieve a new drug against the parasite. © 2015, Tehran University of Medical Sciences (TUMS). All rights reserved.
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