Truncated Jfh1 E2 Protein: Is It Reliable to Be Used in Diagnostic Elisa Test and As a Protein-Based Vaccine Candidate?

Truncated Jfh1 E2 Protein: Is It Reliable to Be Used in Diagnostic Elisa Test and As a Protein-Based Vaccine Candidate? Publisher Pubmed

Bagheri S¹ ; Rahimi P² ; Vahabpour R³ ; Motevalli F² ; Shayestehpour M⁴ ; Amiran MR² ; Yazdani S⁴

Show Affiliations

1. Microbial Biotechnology, Biology Group, Payameh Nour University, Tehran, Iran
2. Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
3. Medical Lab Technology Department, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4. School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Protein and Peptide Letters Published:2017

Abstract

Background: HCV E2 glycoprotein is one of the most attractive proteins for designing an effective vaccine. Deletion of hydrophobic carboxyl-terminal region of this protein is necessary for its secretion, especially when it is expressed in E-coli. In this study we expressed this protein in truncated form and evaluated its application in developing an ELISA test and induction of humoral response in immunized mice. Objectives: The purpose of this study was expression of HCV truncated E2 protein from JFH1 strain in E-coli BL21(DE3) and evaluation of its antigenicity. Methods: Truncated E2 region from HCV genotype 2a (JFH1) was amplified by PCR and cloned into a pET28a (+) vector and was used to transform the E-coli DH5a strain. The recombinant E2 protein was evaluated both in an ELISA test and induction of humoral immunity in mice. Results: Truncated E2 protein was expressed in BL21(DE3). Its specific antibody was detected in serum samples from HCV infected patients. Also, it could elicit a significant humoral immunity in mice. Conclusion: Truncated form of E2 protein which has been expressed in E-coli could be used as an effective antigen both in diagnostic tests such as ELISA and also, as a protein-based vaccine candidate. © 2017 Bentham Science Publishers.

Style	Citing Format
MLA	Bagheri S, et al.. "Truncated Jfh1 E2 Protein: Is It Reliable to Be Used in Diagnostic Elisa Test and As a Protein-Based Vaccine Candidate?." Protein and Peptide Letters, vol. 24, no. 12, 2017, pp. 1179-1184.
APA	Bagheri S, Rahimi P, Vahabpour R, Motevalli F, Shayestehpour M, Amiran MR, Yazdani S (2017). Truncated Jfh1 E2 Protein: Is It Reliable to Be Used in Diagnostic Elisa Test and As a Protein-Based Vaccine Candidate?. Protein and Peptide Letters, 24(12), 1179-1184.
Chicago	Bagheri S, Rahimi P, Vahabpour R, Motevalli F, Shayestehpour M, Amiran MR, Yazdani S. "Truncated Jfh1 E2 Protein: Is It Reliable to Be Used in Diagnostic Elisa Test and As a Protein-Based Vaccine Candidate?." Protein and Peptide Letters 24, no. 12 (2017): 1179-1184.
Harvard	Bagheri S et al. (2017) 'Truncated Jfh1 E2 Protein: Is It Reliable to Be Used in Diagnostic Elisa Test and As a Protein-Based Vaccine Candidate?', Protein and Peptide Letters, 24(12), pp. 1179-1184.
Vancouver	Bagheri S, Rahimi P, Vahabpour R, Motevalli F, Shayestehpour M, Amiran MR, et al.. Truncated Jfh1 E2 Protein: Is It Reliable to Be Used in Diagnostic Elisa Test and As a Protein-Based Vaccine Candidate?. Protein and Peptide Letters. 2017;24(12):1179-1184.
BibTex	@article{ author = {Bagheri S and Rahimi P and Vahabpour R and Motevalli F and Shayestehpour M and Amiran MR and Yazdani S}, title = {Truncated Jfh1 E2 Protein: Is It Reliable to Be Used in Diagnostic Elisa Test and As a Protein-Based Vaccine Candidate?}, journal = {Protein and Peptide Letters}, volume = {24}, number = {12}, pages = {1179-1184}, year = {2017} }
RIS	TY - JOUR AU - Bagheri S AU - Rahimi P AU - Vahabpour R AU - Motevalli F AU - Shayestehpour M AU - Amiran MR AU - Yazdani S TI - Truncated Jfh1 E2 Protein: Is It Reliable to Be Used in Diagnostic Elisa Test and As a Protein-Based Vaccine Candidate? JO - Protein and Peptide Letters VL - 24 IS - 12 SP - 1179 EP - 1184 PY - 2017 ER -

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