Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Effect of Low-Level Laser Irradiation on the Function of Glycated Catalase Publisher



Mirmiranpour H1 ; Nosrati FS2 ; Sobhani SO2, 3 ; Takantape SN4 ; Amjadi A2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Endocrinology and Metabolism Research Center (EMRC), Valiasr Hospital, School of Medicine, Tehran University of Medical Science, Tehran, Iran
  2. 2. Laser and medical Physics lab, Department of Physics, Sharif University of Technology, Tehran, Iran
  3. 3. Department of Energy Engineering, Sharif University of Technology, Tehran, Iran
  4. 4. Department of Aerospace Engineering, Sharif University of Technology, Tehran, Iran

Source: Journal of Lasers in Medical Sciences Published:2018


Abstract

Introduction: The aim of this work is to evaluate the effect of low-level laser irradiation (LLLI), by lasers with different wavelengths, on glycated catalase enzyme in vitro experimentally. Methods: This is done by measuring the activity and structure properties of glycated catalase enzyme. The structure properties were evaluated with circular dichroism (CD) and fluoroscopy methods. Three continuous wave (CW) lasers in the visible spectrum (λ = 450, 530, 638 nm) and a 100-ns pulsed laser in the infrared spectrum (λ = 905 nm) were chosen for comparison. For the infrared laser, same effects have been investigated for different energy doses. The effect of photon energy (hλ) at different wavelengths was measured on activity, CD, and fluoroscopy properties of catalase, and compared with the control group (samples without irradiation). The energy intensity of laser should not exceed 0.1 J/cm2. Experiments were performed on glycated catalase between 2 to 16 weeks after glycation of catalase. The LLLI effect was also investigated on the samples, by comparing the catalase activity, CD and fluoroscopy for different wavelengths. Results: Our results indicated, the decrease in catalase activity as a function of glycation time (weeks) for all samples, and a slight increase on its activity by different laser wavelengths irradiation for any fixed period of glycation time. Finally, the catalase activity has been increased as the laser's photon energy (hλ) intensified. More specifically, the blue laser (λ = 450 nm) had the most and the red laser (λ = 638 nm) had the least effect, and the green laser (λ = 530 nm) had the medium effect on catalase activity as well. Furthermore, pulsed laser had an additional effect by increasing energy dosage. Conclusion: As we expected in all experiments, an increase in the catalase activity was coincident with a decrease in the catalase fluoroscopy and CD parameters. © 2012 J Lasers Med Sci Powered by Open Journal Systems.
Related Docs
1. Intravenous Laser Wavelength Radiation Effect on Lcat, Pon1, Catalase, and Frap in Diabetic Rats, Lasers in Medical Science (2020)
2. Effect of Pulsed Electric Field on Diabetes-Induced Glycated Enzyme, Oxidative Stress, and Inflammatory Markers in Vitro and in Vivo, Journal of Physics D: Applied Physics (2021)
3. Effects of Quercetin and Low-Level Laser on Oxidative and Inflammatory Factors Among Patients With Type 2 Diabetes Mellitus and Mild Cognitive Impairment, Bioactive Compounds in Health and Disease (2022)
4. The Effect of Cold Atmospheric Plasma on Diabetes-Induced Enzyme Glycation, Oxidative Stress, and Inflammation; in Vitro and in Vivo, Scientific Reports (2019)
5. L-Lysine Supplementation Improved Glycemic Control, Decreased Protein Glycation, and Insulin Resistance in Type 2 Diabetic Patients, International Journal of Diabetes in Developing Countries (2021)
6. Co-Administration of “L-Lysine, Vitamin C, and Zinc” Increased the Antioxidant Activity, Decreased Insulin Resistance, and Improved Lipid Profile in Streptozotocin-Induced Diabetic Rats, Biomedicine and Pharmacotherapy (2024)
7. L-Lysine Protects C2c12 Myotubes and 3T3-L1 Adipocytes Against High Glucose Damages and Stresses, PLoS ONE (2019)
8. The Preventive Effect of L-Lysine on Lysozyme Glycation in Type 2 Diabetes, Acta Medica Iranica (2016)