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Identification of Serum N-Glycoproteins As a Biological Correlate Underlying Chronic Stress Response in Mice Publisher Pubmed



Mahmoud ME1 ; Rehan IF2 ; Ahmed KD3 ; Abdelrahman A4 ; Mohammadi S5, 6 ; Abouelnaga AF7 ; Youssef M8 ; Diab HM9 ; Salman D10 ; Elnagar A3 ; Mohammed HH11 ; Shanab O12 ; Ibrahim RM13 ; Ahmed EKH2 Show All Authors
Authors
  1. Mahmoud ME1
  2. Rehan IF2
  3. Ahmed KD3
  4. Abdelrahman A4
  5. Mohammadi S5, 6
  6. Abouelnaga AF7
  7. Youssef M8
  8. Diab HM9
  9. Salman D10
  10. Elnagar A3
  11. Mohammed HH11
  12. Shanab O12
  13. Ibrahim RM13
  14. Ahmed EKH2
  15. Hesham AL14
  16. Gupta A15
Show Affiliations
Authors Affiliations
  1. 1. Department of Animal Behavior and Husbandry, Sohag University, Sohag, 82524, Egypt
  2. 2. Department of Animal Behavior and Husbandry, South Valley University, Qena, 83523, Egypt
  3. 3. Department of Biochemistry, Zagazig University, El-Zeraa Street, 114, Zagazig, 44511, Egypt
  4. 4. Department of Physiology, Sohag University, Sohag, 82524, Egypt
  5. 5. Department of Tissue Engineering and Applied Cell Sciences, University of Medical Sciences, Tehran, 1985711151, Iran
  6. 6. Biointerfaces Institute, McMaster University, 1280 Main St W, Hamilton, L8S 0A3, ON, Canada
  7. 7. Department of Husbandry and Development of Animal Wealth, Mansoura University, Mansoura, 35516, Egypt
  8. 8. Department of Animal Physiology, South Valley University, Qena, 83523, Egypt
  9. 9. Department of Animal Hygiene, South Valley University, Qena, 83523, Egypt
  10. 10. Department of Animal Medicine, Sohag University, Sohag, 82524, Egypt
  11. 11. Department of Veterinary Public Health, Zagazig University, El-Zeraa Street, 114, Zagazig, 44511, Egypt
  12. 12. Department of Biochemistry, South Valley University, Qena, 83523, Egypt
  13. 13. Clinical Laboratory Diagnosis, Department of Animal Medicine, South Valley University, Qena, 83523, Egypt
  14. 14. Genetics Department, Assiut University, Assiut, 71516, Egypt
  15. 15. Department of Zoology, Sri Avadh Raj Singh Smarak Degree College, Gonda, India

Source: Molecular Biology Reports Published:2019


Abstract

Glycosylation is a post-translational protein modification in eukaryotes and plays an important role in controlling several diseases. N-glycan structure is emerging as a new paradigm for biomarker discovery of neuropsychiatric disorders. However, the relationship between N-glycosylation pattern and depression is not well elucidated to date. This study aimed to explore whether serum N-glycan structures are altered in depressive-like behavior using a stress based mouse model. We used two groups of BALB/c mice; (i) treated group exposed to chronic unpredictable mild stress (CUMS) as a model of depression, and (ii) control group. Behavioral tests in mice (e.g., sucrose preference test, forced swimming test, and fear conditioning test) were used to evaluate the threshold level to which mice displayed a depressive-like phenotype. Serum N-glycans were analyzed carefully using glycoblotting followed by Matrix-assisted laser desorption ionization-time of flight/mass spectrometry (MALDI-TOF/MS) to exhibit N-glycan expression levels and to illustrate the changes in the N-glycome profile. N-glycan expression levels were commonly altered in the depressive-like model and correlated well with the behavioral data. Our results indicated that sialylated N-glycan was identified as a biomarker associated with depressive symptoms, which may have utility as a candidate biomarker for the clinical diagnosis and monitoring of depression. © Springer Nature B.V. 2019.
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