Protein Profiling of Hirudo Orientalis During Different Seasons for Obtaining Accurate Results in Leech Therapy

Protein Profiling of Hirudo Orientalis During Different Seasons for Obtaining Accurate Results in Leech Therapy Publisher

Amani L¹ ; Ardakani MM¹ ; Motamed N² ; Malek M³

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1. Department of Traditional Pharmacy, School of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
3. Department of Biology, School of Zoology, College of Science, University of Tehran, Tehran, Iran

Source: Current Proteomics Published:2023

Abstract

Objective: Leech therapy has been used for centuries as a recommended approach to cure several diseases, such as; psoriasis, arthrosclerosis, urinary tract diseases, and wound healing. The present study aimed to analyze the number, quantity, and distribution differences of medicinal leech (Hirudo orientalis) proteins throughout various seasons and in laboratory conditions as well. Methods: Protein profiling of salivary gland secretion from leech was studied by SDS-PAGE and 2D Electrophoresis on the proteins with the molecular weight range of 5 - 250 KDa in the lyophilized salivary gland secretion (SGS) during the seasons of summer and winter, and also in the laboratory conditions. Results: Our results indicated differences in the number and quality of leech saliva proteins in different seasons. We observed a higher number of proteins in summer than in winter. These results demonstrated the presence of Calin and Manillase in summer and Hyaluronidase and Collagenase in winter. Conclusion: This study could help us in choosing the best and most favorable conditions for using H. orientalis proteins for the treatment of different diseases. © 2023 Bentham Science Publishers.

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Style	Citing Format
MLA	Amani L, et al.. "Protein Profiling of Hirudo Orientalis During Different Seasons for Obtaining Accurate Results in Leech Therapy." Current Proteomics, vol. 20, no. 3, 2023, pp. 184-191.
APA	Amani L, Ardakani MM, Motamed N, Malek M (2023). Protein Profiling of Hirudo Orientalis During Different Seasons for Obtaining Accurate Results in Leech Therapy. Current Proteomics, 20(3), 184-191.
Chicago	Amani L, Ardakani MM, Motamed N, Malek M. "Protein Profiling of Hirudo Orientalis During Different Seasons for Obtaining Accurate Results in Leech Therapy." Current Proteomics 20, no. 3 (2023): 184-191.
Harvard	Amani L et al. (2023) 'Protein Profiling of Hirudo Orientalis During Different Seasons for Obtaining Accurate Results in Leech Therapy', Current Proteomics, 20(3), pp. 184-191.
Vancouver	Amani L, Ardakani MM, Motamed N, Malek M. Protein Profiling of Hirudo Orientalis During Different Seasons for Obtaining Accurate Results in Leech Therapy. Current Proteomics. 2023;20(3):184-191.
BibTex	@article{ author = {Amani L and Ardakani MM and Motamed N and Malek M}, title = {Protein Profiling of Hirudo Orientalis During Different Seasons for Obtaining Accurate Results in Leech Therapy}, journal = {Current Proteomics}, volume = {20}, number = {3}, pages = {184-191}, year = {2023} }
RIS	TY - JOUR AU - Amani L AU - Ardakani MM AU - Motamed N AU - Malek M TI - Protein Profiling of Hirudo Orientalis During Different Seasons for Obtaining Accurate Results in Leech Therapy JO - Current Proteomics VL - 20 IS - 3 SP - 184 EP - 191 PY - 2023 ER -

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