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Green Synthesis and Characterization of Mnco 2 O 4 /Co 2 Mn 3 O 8 Ceramic Nanocomposites and Investigation of Their Cytotoxicity on the 4T1 Cells Publisher



Goudarzi M1 ; Salavatiniasari M1 ; Azizi M2 ; Yazdian F3 ; Birhanu G4, 5
Authors
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Authors Affiliations
  1. 1. Institute of Nano Science and Nano Technology, University of Kashan, P. O. Box. 87317-51167, Kashan, Iran
  2. 2. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
  3. 3. Department of Life Science Engineering, Faculty of New Science and Technology, Tehran, Iran
  4. 4. Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, International Campus (TUMS-IC), Tehran, Iran
  5. 5. School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia

Source: Composites Part B: Engineering Published:2019


Abstract

In this study, MnCo 2 O 4 /Co 2 Mn 3 O 8 nanocomposites was synthesized for the first time by thermal decomposition of a natural complex involving Mn(NO 3 ) 2 , Co(NO 3 ) 2 and cochineal powder. Cochineal powder was dissolved in water and two aqua solutions of Mn(NO 3 ) 2 and Co(NO 3 ) 2 were added to it. The reaction of Co 2+ and Mn 2+ with functional groups of cochineal produced a stable complex. With heating this natural complex, MnCo 2 O 4 /Co 2 Mn 3 O 8 nanocomposites were successfully obtained. MTT cytotoxicity assay was conducted to determine the anti-cancer effect of the MnCo 2 O 4 /Co 2 Mn 3 O 8 nanocomposites synthesized against 4T1 cell lines. The mechanism of cell death was evaluated by acridine orange/ethidium bromide staining methods, DAPI staining, flow cytometry. To know the effect of the MnCo 2 O 4 /Co 2 Mn 3 O 8 nanocomposites on oxidative stress, the reactive oxygen species (ROS) generation was measured using the H 2 DCF-DA assay. The results show that, the MnCo 2 O 4 /Co 2 Mn 3 O 8 nanocomposites significantly reduced the survival percentage of the 4T1 cells of the murine invasive breast cancer with IC 50 of 2 mg/ml. The dominant mechanism of cell death was apoptosis, along with an increase in cellular oxidative stress. Therefore, the nanocomposites synthesized here can potential be used as a chemotherapeutic agent against invasive breast cancer cells. © 2019 Elsevier Ltd
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