Proteomics Study of Extremely Low Frequency Electromagnetic Field (50 Hz) on Human Neuroblastoma Cells



Hasanzadeh H¹ ; Rezaietavirani M² ; Seyyedi S³ ; Emadi A² Show Affiliations
Source: Koomesh Published:2015

Abstract

Introduction: Extremely low frequency electromagnetic field (ELF-EMF) is one of common environmental factors in today’s modern life. In recent years, a special interest has been directed toward the possible effects of ELF-EMFs on biological systems. Since the mechanism underlying the ELF-EMFs’ biological effects are still unknown, we decided to conduct an in vitro study to understand the possible mechanisms. Material and methods: SH-SY5Y- human neuroblastoma cell line were placed in the EL-EM Field with the intensity of 1 mT and frequency of 50 Hz and for a period of 4 hours/day, in 2 days period. The cell morphology and protein expression were measured by using proteomic methods. Statistical analysis was performed by using Non Linear Spot Progenesis Same software. Results and discussion: Our results showed that ELF-EMF could likely change the cell morphology and proliferation. Conclusion: Statistical analysis in our study showed that ELF-EMF with a frequency of 50Hz and intensity of 1mT could significantly change the protein expression (P ≤ 0.05). © 2015, Semnan University of Medical Sciences. All rights reserved.