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Proteomics Study of Extremely Low Frequency Electromagnetic Field (50 Hz) on Human Neuroblastoma Cells



Hasanzadeh H1 ; Rezaietavirani M2 ; Seyyedi S3 ; Emadi A2
Authors
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Authors Affiliations
  1. 1. Cancer Research Center and Department of Medical Physics, Semnan University of Medical Sciences, Semnan, Iran
  2. 2. Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Dept. Of Medical Genetics, Tehran University of Medical Sciences, International Campus, Tehran, Iran

Source: Koomesh Published:2015

Abstract

Introduction: Extremely low frequency electromagnetic field (ELF-EMF) is one of common environmental factors in today’s modern life. In recent years, a special interest has been directed toward the possible effects of ELF-EMFs on biological systems. Since the mechanism underlying the ELF-EMFs’ biological effects are still unknown, we decided to conduct an in vitro study to understand the possible mechanisms. Material and methods: SH-SY5Y- human neuroblastoma cell line were placed in the EL-EM Field with the intensity of 1 mT and frequency of 50 Hz and for a period of 4 hours/day, in 2 days period. The cell morphology and protein expression were measured by using proteomic methods. Statistical analysis was performed by using Non Linear Spot Progenesis Same software. Results and discussion: Our results showed that ELF-EMF could likely change the cell morphology and proliferation. Conclusion: Statistical analysis in our study showed that ELF-EMF with a frequency of 50Hz and intensity of 1mT could significantly change the protein expression (P ≤ 0.05). © 2015, Semnan University of Medical Sciences. All rights reserved.