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Comparing Four Laboratory Three-Parent Techniques to Construct Human Aged Non-Surrounded Nucleolus Germinal Vesicle Oocytes: A Case-Control Study Publisher



Darbandi S1 ; Darbandi M1 ; Agarwal A2 ; Khorshid HRK3 ; Sadeghi MR1 ; Esteves SC4 ; Sengupta P5 ; Dutta S5 ; Fathi Z1 ; Zeraati H6 ; Akhondi MM1
Authors
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Authors Affiliations
  1. 1. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
  2. 2. American Center for Reproductive Medicine, Cleveland Clinic, Cleveland, OH, United States
  3. 3. Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran
  4. 4. Androfert, Andrology and Human Reproduction Clinic, Campinas, Brazil
  5. 5. Department of Physiology, Faculty of Medicine, Mahsa University, Selangor, Malaysia
  6. 6. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: International Journal of Reproductive BioMedicine Published:2020


Abstract

Background: The three-parent assisted reproductive technique may increase oocyte competence. Objective: In this case-control study, the suitability of germinal vesicle transfer (GVT), synchronous ooplasmic transfer (sOT), asynchronous ooplasmic transfer using cryopreserved MII oocyte (caOT), and asynchronous ooplasmic transfer using waste MII oocyte (waOT) for maturation of the human-aged non-surrounded nucleolus germinal vesicle-stage (NSN-GV) oocyte were investigated. Materials and Methods: NSN-GV oocytes were subjected to four methods: group A (GVT), B (sOT), C (caOT) D (waOT), and E (Control). The fusion rates, MI, MII, ICSI observations and cleavage at 2-cell, 4-cell, and 8-cell stages were compared in the groups. Results: In GVT, none of the oocytes fused. In sOT, all oocytes fused, 20 achieved the MI, 14 progressed to MII, 8 fertilized, 6 cleaved and 5, 4, and 3 achieved the 2cells, 4-cells and 8-cells, respectively. In caOT, all oocytes fused and achieved the MI, 8 progressed to MII and fertilized, 6 cleaved and 6, 5, and 5 achieved the 2-cells, 4cells, and 8-cells respectively. In waOT, all oocytes fused, 5 and 3 progressed to MI and MII, respectively, but only one fertilized, cleaved and reached a 4-cells stage. In group E, 6 and 2 oocytes progressed to MI and MII, respectively, and only one fertilized but arrested at the zygote stage. caOT had the highest survival rate when compared to sOT (p = 0.04), waOT (p = 0.002), and control (p = 0.001). Conclusion: The caOT method was beneficial over sOT, waOT, and GVT in supplementing the developmental capacity of human-aged NSN-GV oocytes. © 2020, Research and Clinical Center for Infertitlity. All rights reserved.
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