Immobilising Effect of Ruta Graveolens L. on Human Spermatozoa: Coumarin Compounds Are Involved

Immobilising Effect of Ruta Graveolens L. on Human Spermatozoa: Coumarin Compounds Are Involved Publisher Pubmed

Naghibi Harat Z¹ ; Lakpour N² ; Sadeghipoor HR³ ; Kamalinejad M⁴ ; Eshraghian MR⁵ ; Naghibi B⁶ ; Akhondi MM⁷ ; Binaafar S⁷ ; Sadeghi MR⁸

Show Affiliations

1. IVF Center, Arash Women Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
3. Physiology Department, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5. Biostatics Department, School of Health, Tehran University of Medical Sciences, Tehran, Iran
6. Department of Toxicology and Pharmacology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
7. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
8. Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Source: Andrologia Published:2015

Abstract

This study was designed to find out Ruta graveolens L. functional components, which have immobilisation effect on human spermatozoa for contraceptive use. A five-step fractionation method was used to derive different components from rue aqueous extract by using hexane, chloroform, ethanol, acetone and ultrapure water. Gas chromatography-mass spectrophotometery (GC-MS) of all fractions and the aqueous extract were performed to determine the chemical components. The immobilisation assay and membrane integrity test were also performed with four different coumarins, which were found in GC-MS in a concentration of 10 μm. Hexane, chloroform, acetone and ethanol fractions could significantly decrease motility of sperms within the first and the second hours. Hexane fraction had also significant immediate effect. The aqueous fraction had no effect on sperm motility. Meanwhile, GC-MS revealed that aqueous extract and effective fractions had similar coumarin compounds. We performed the immobilisation assay on four different coumarins, which were found in GC-MS in a concentration of 10 μm. Reduction of sperm motility was only significant for xanthotoxin. In the sperm viability and membrane integrity tests, hexane and ethanolic fractions could impair sperm vitality significantly, in contrast to coumarins. These results indicated that a part of immobilising effect of rue could be due to its coumarins. The possible mechanism could be blocking of spermatozoa potassium channels. © 2015 Blackwell Verlag GmbH.

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Style	Citing Format
MLA	Naghibi Harat Z, et al.. "Immobilising Effect of Ruta Graveolens L. on Human Spermatozoa: Coumarin Compounds Are Involved." Andrologia, vol. 47, no. 10, 2015, pp. 1183-1189.
APA	Naghibi Harat Z, Lakpour N, Sadeghipoor HR, Kamalinejad M, Eshraghian MR, Naghibi B, Akhondi MM, Binaafar S, Sadeghi MR (2015). Immobilising Effect of Ruta Graveolens L. on Human Spermatozoa: Coumarin Compounds Are Involved. Andrologia, 47(10), 1183-1189.
Chicago	Naghibi Harat Z, Lakpour N, Sadeghipoor HR, Kamalinejad M, Eshraghian MR, Naghibi B, Akhondi MM, Binaafar S, Sadeghi MR. "Immobilising Effect of Ruta Graveolens L. on Human Spermatozoa: Coumarin Compounds Are Involved." Andrologia 47, no. 10 (2015): 1183-1189.
Harvard	Naghibi Harat Z et al. (2015) 'Immobilising Effect of Ruta Graveolens L. on Human Spermatozoa: Coumarin Compounds Are Involved', Andrologia, 47(10), pp. 1183-1189.
Vancouver	Naghibi Harat Z, Lakpour N, Sadeghipoor HR, Kamalinejad M, Eshraghian MR, Naghibi B, et al.. Immobilising Effect of Ruta Graveolens L. on Human Spermatozoa: Coumarin Compounds Are Involved. Andrologia. 2015;47(10):1183-1189.
BibTex	@article{ author = {Naghibi Harat Z and Lakpour N and Sadeghipoor HR and Kamalinejad M and Eshraghian MR and Naghibi B and Akhondi MM and Binaafar S and Sadeghi MR}, title = {Immobilising Effect of Ruta Graveolens L. on Human Spermatozoa: Coumarin Compounds Are Involved}, journal = {Andrologia}, volume = {47}, number = {10}, pages = {1183-1189}, year = {2015} }
RIS	TY - JOUR AU - Naghibi Harat Z AU - Lakpour N AU - Sadeghipoor HR AU - Kamalinejad M AU - Eshraghian MR AU - Naghibi B AU - Akhondi MM AU - Binaafar S AU - Sadeghi MR TI - Immobilising Effect of Ruta Graveolens L. on Human Spermatozoa: Coumarin Compounds Are Involved JO - Andrologia VL - 47 IS - 10 SP - 1183 EP - 1189 PY - 2015 ER -

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