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A Sensitive Biosensor for Acrylamide Detection Based on Polyaniline and Au Nanoparticles Using Fft Admittance Voltammetry



Norouzi P1, 2 ; Larijani B3 ; Bidhendi ME4 ; Eshraghi M1 ; Ebrahimi M1
Authors
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Authors Affiliations
  1. 1. Center of Excellence in Electrochemistry, School of Chemistry, College of Science, University of Tehran, Tehran, Iran
  2. 2. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Endocrinology & Metabolism Research Center, Endocrinology & Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Graduate Faculty of Environment, University of Tehran, P.O. Box 14155-6135, Tehran, Iran

Source: Analytical and Bioanalytical Electrochemistry Published:2018

Abstract

A new electrochemical detection system was developed based on combination of a biosensor and Fast Fourier transform Admittance Voltammetry (FFTAV), which used for the sensitive detection of acrylamide. By effective self-assembling process, the biosensor was prepared of thiol functionalized single-stranded DNA (ssDNA) on gold nanoparticles, which deposited on a gold electrode decorated with polyaniline. The acrylamide has the ability to form a single complex with ssDNAs, which was linked on the biosensor surface, and changed the admittance of the electrode. Therefore, the concentration of acrylamide was detected directly by the change of admittance of the electrode. The biosensor was characterized by Scanning electron microscope-based (SEM), electrochemical impedance spectroscopy copy (EIS) and cyclic voltammetry. Under optimal conditions, the linear dynamic range of the acrylamide was 5.0×10-10 M to 2.0×10-7 M (r2 = 0.988) limits of detection of 5.0×10-11 M. The data showed that the electrochemical biosensor could detect acrylamide rapidly and accurately. Moreover, the proposed method demonstrated acceptable sensitivity and long-term stability. © 2018 by CEE (Center of Excellence in Electrochemistry).