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Evaluation of Biofilm Formation Among Klebsiella Pneumoniae Isolates and Molecular Characterization by Eric-Pcr Publisher



Seifi K1 ; Kazemian H2 ; Heidari H3 ; Rezagholizadeh F2 ; Saee Y4 ; Shirvani F1 ; Houri H5
Authors
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Authors Affiliations
  1. 1. Pediatric Infections Research Center, Mofid Children’s Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Department of Medical Microbiology School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Bacteriology and Virology School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  4. 4. Department of Microbiology Islamic, Azad University Pharmaceutical Branch, Tehran, Iran
  5. 5. Department of Microbiology School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Jundishapur Journal of Microbiology Published:2016


Abstract

Background: Klebsiella pneumoniae is among the most frequently recovered etiologic agents from nosocomial infections. This opportunistic pathogen can generate a thick layer of biofilm as one of its important virulence factors enabling the bacteria to attach to living or abiotic surfaces which contributes to drug resistance. Objectives: The resistance of biofilm-mediated infections to effective chemotherapy has adverse effects on patient outcomes and survival. Therefore the aim of the present study was to evaluate the biofilm-formation capacity of clinical K. pneumoniae isolates and to perform a molecular characterization using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) to determine the dominant biofilm-producing genotype. Patients and Methods: In the present study 94 K. pneumoniae isolates were obtained from two hospitals in Tehran Iran. Biofilm formation was assayed by a modified procedure then ERIC-PCR was carried out. Results: The distributions of the clinical specimens used in this study were 61.7% from urine 18.1% from wounds 11.7% from sputum and 8.5% from blood. Among these isolates 33% formed fully established biofilms 52.1% were categorized as moderately biofilm-producing 8.5% formed weak biofilms and 6.4% were non-biofilm-producers. Genotyping of K. pneumoniae revealed 31 different ERIC types. Biofilm-formation ability in a special ERIC type was not observed. Conclusions: Our results indicated that an enormous proportion of K. pneumoniae isolated from sputum and surgical-wound swabs produced fully established biofilms. It is reasonable to assume the existence of a relationship between the site of infection and the formation of biofilm. A high level of genetic diversity among the K. pneumoniae strains was observed. © 2016 Ahvaz Jundishapur University of Medical Sciences.
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