Efficiency of Nested-Pcr in Detecting Asymptomatic Cases Toward Malaria Elimination Program in an Endemic Area of Iran

Efficiency of Nested-Pcr in Detecting Asymptomatic Cases Toward Malaria Elimination Program in an Endemic Area of Iran

Turki H^{1, 2} ; Raeisi A³ ; Malekzadeh K² ; Ghanbarnejad A⁴ ; Zoghi S⁵ ; Yeryan M¹ ; Abedi Nejad M² ; Mohseni F² ; Shekari M²

Show Affiliations

1. Infectious and Tropical diseases, Research Center Hormozgan University of Medical Sciences, Bandar Abbas, Iran
2. Molecular Medicine Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
3. National Programme Manager for Malaria Control, Ministry of Health and Medical Education, Tehran, Iran
4. Social Determinants in Health Promotion Research Center, Hormozgan University of Medical Sciences, Bandar-Abbas, Iran
5. Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Parasitology Published:2015

Abstract

Background: The aim of this study was to detect low parasite and asymptomatic malaria infections by means of three malaria diagnostic tests, in a low transmission region of Minab district, Hormozgan Province, southern Iran. Methods: Blood samples of 200 healthy volunteers from Bagh-e-Malek area were evaluated using microscopic, rapid diagnostic tests (RDT) and nested-PCR to inspect malaria parasite. Results: The results showed no Plasmodium parasite in subjects by means of microscopy and RDT. However, 3 P. vivax positive samples (1.5%) were discovered by Nested-PCR while microscopy and RDT missed the cases. Conclusion: Microscopy as the gold standard method and RDT correctly identified 98.5% of cases, and molecular analysis is sensitive and reliable, especially in the detection of asymptomatic infections for active case surveillance. Regarding the existence of asymptomatic malaria in endemic area of Hormozgan, Iran, nested- PCR could be considered as a sensitive tool to interrupt malaria transmission in the country, beside the microscopic and RDT methods. © 2015, Iran J Parasitol. All Rights Reserved.

2. Detection of Malaria Using Blood Smear by Light Microscopy, Rdt and Nested-Pcr for Suspected Patients in South-Eastern Iran, Gene Reports (2021)

3. Asymptomatic Malaria and Its Challenges in the Malaria Elimination Program in Iran: A Systematic Review, Journal of Arthropod-Borne Diseases (2017)

4. Situation of Asymptomatic Malaria Among Iranian Native and Afghan and Pakistani Immigrants in a Malarious Area Under the National Malaria Elimination Program of Iran, Iranian Journal of Parasitology (2020)

5. Detection of Mixed-Species Infections of Plasmodium Falciparum and Plasmodium Vivax by Nested Pcr and Rapid Diagnostic Tests in Southeastern Iran, American Journal of Tropical Medicine and Hygiene (2015)

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Style	Citing Format
MLA	Turki H, et al.. "Efficiency of Nested-Pcr in Detecting Asymptomatic Cases Toward Malaria Elimination Program in an Endemic Area of Iran." Iranian Journal of Parasitology, vol. 10, no. 1, 2015, pp. 39-45.
APA	Turki H, Raeisi A, Malekzadeh K, Ghanbarnejad A, Zoghi S, Yeryan M, Abedi Nejad M, Mohseni F, Shekari M (2015). Efficiency of Nested-Pcr in Detecting Asymptomatic Cases Toward Malaria Elimination Program in an Endemic Area of Iran. Iranian Journal of Parasitology, 10(1), 39-45.
Chicago	Turki H, Raeisi A, Malekzadeh K, Ghanbarnejad A, Zoghi S, Yeryan M, Abedi Nejad M, Mohseni F, Shekari M. "Efficiency of Nested-Pcr in Detecting Asymptomatic Cases Toward Malaria Elimination Program in an Endemic Area of Iran." Iranian Journal of Parasitology 10, no. 1 (2015): 39-45.
Harvard	Turki H et al. (2015) 'Efficiency of Nested-Pcr in Detecting Asymptomatic Cases Toward Malaria Elimination Program in an Endemic Area of Iran', Iranian Journal of Parasitology, 10(1), pp. 39-45.
Vancouver	Turki H, Raeisi A, Malekzadeh K, Ghanbarnejad A, Zoghi S, Yeryan M, et al.. Efficiency of Nested-Pcr in Detecting Asymptomatic Cases Toward Malaria Elimination Program in an Endemic Area of Iran. Iranian Journal of Parasitology. 2015;10(1):39-45.
BibTex	@article{ author = {Turki H and Raeisi A and Malekzadeh K and Ghanbarnejad A and Zoghi S and Yeryan M and Abedi Nejad M and Mohseni F and Shekari M}, title = {Efficiency of Nested-Pcr in Detecting Asymptomatic Cases Toward Malaria Elimination Program in an Endemic Area of Iran}, journal = {Iranian Journal of Parasitology}, volume = {10}, number = {1}, pages = {39-45}, year = {2015} }
RIS	TY - JOUR AU - Turki H AU - Raeisi A AU - Malekzadeh K AU - Ghanbarnejad A AU - Zoghi S AU - Yeryan M AU - Abedi Nejad M AU - Mohseni F AU - Shekari M TI - Efficiency of Nested-Pcr in Detecting Asymptomatic Cases Toward Malaria Elimination Program in an Endemic Area of Iran JO - Iranian Journal of Parasitology VL - 10 IS - 1 SP - 39 EP - 45 PY - 2015 ER -

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