Gene Expression and Levels of Il-6 and Tnfα in Pbmcs Correlate With Severity and Functional Class in Patients With Chronic Heart Failure Publisher Pubmed



Eskandari V¹ ; Amirzargar AA¹ ; Mahmoudi MJ² ; Rahnemoon Z³ ; Rahmani F^{4, 5} ; Sadati S¹ ; Rahmati Z¹ ; Gorzin F¹ ; Hedayat M^{6, 7} ; Rezaei N^{1, 4, 5} Show Affiliations
Source: Irish Journal of Medical Science Published:2018

Abstract

Background: Evidence shows that proinflammatory cytokines are important determinants of assessment of severity and prognosis of chronic heart failure (CHF). Aims: We investigated whether peripheral expression of the proinflammmatory factors, TNF-α and IL-6 can predict variable of clinical assessment of patients with CHF. Methods: In this report, we used real-time PCR assay to compare relative gene expression of TNFα and IL-6 in PBMC from CHF patients with various heart diseases (n = 42, EF < 45%, NYHA I to IV) and matched healthy control subjects (n = 42).We also determined the TNFα and IL-6 concentrations of cell culture supernatant of PBMCs with ELISA. Results: There was a significant negative correlation between gene expression of TNFα and LVEF(r = 0.4, p < 0.05). Patients with CHF had increased gene expression of TNFα and IL-6 in PBMCs (p < 0.05). They also had elevated the supernatant levels of these cytokines in cultured PBMCs (p < 0.001). Levels of TNFα and IL-6 were increased in ischemic heart disease compared to non-ischemic heart disease. There was a positive correlation between TNFα and IL-6 levels in CHF patients and severity of CHF in patients. Levels of these cytokines were higher in patients with NYHA III–IV than in NYHA I–II and normal subjects. Conclusions: Results of this study indicate that peripheral expression of proinflammatory cytokines, TNF-α and IL-6, is important indicators of severity and prognosis in patients with chronic heart disease. © 2017, Royal Academy of Medicine in Ireland.