Comparison of Different Methods for Erythroid Differentiation in the K562 Cell Line

Comparison of Different Methods for Erythroid Differentiation in the K562 Cell Line Publisher Pubmed

Shariati L¹ ; Modaress M² ; Khanahmad H^{2, 3} ; Hejazi Z² ; Tabatabaiefar MA^{2, 3} ; Salehi M² ; Modarressi MH^{1, 4}

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1. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3. Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran
4. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Biotechnology Letters Published:2016

Abstract

Objective: To compare methods for erythroid differentiation of K562 cells that will be promising in the treatment of beta-thalassemia by inducing γ-globin synthesis. Results: Cells were treated separately with: RPMI 1640 medium without glutamine, RPMI 1640 medium without glutamine supplemented with 1 mM sodium butyrate, RPMI 1640 medium supplemented with 1 mM sodium butyrate, 25 µg cisplatin/ml, 0.1 µg cytosine arabinoside/ml. The highest differentiation (84 %) with minimum toxicity was obtained with cisplatin at 15 µg /ml. Real-time RT-PCR showed that expression of the γ-globin gene was significantly higher in the cells differentiated with cisplatin compared to undifferentiated cells (P < 0.001). Conclusions: Cisplatin is useful in the experimental therapy of ß-globin gene defects and can be considered for examining the basic mechanism of γ-reactivation. © 2016, Springer Science+Business Media Dordrecht.

Style	Citing Format
MLA	Shariati L, et al.. "Comparison of Different Methods for Erythroid Differentiation in the K562 Cell Line." Biotechnology Letters, vol. 38, no. 8, 2016, pp. 1243-1250.
APA	Shariati L, Modaress M, Khanahmad H, Hejazi Z, Tabatabaiefar MA, Salehi M, Modarressi MH (2016). Comparison of Different Methods for Erythroid Differentiation in the K562 Cell Line. Biotechnology Letters, 38(8), 1243-1250.
Chicago	Shariati L, Modaress M, Khanahmad H, Hejazi Z, Tabatabaiefar MA, Salehi M, Modarressi MH. "Comparison of Different Methods for Erythroid Differentiation in the K562 Cell Line." Biotechnology Letters 38, no. 8 (2016): 1243-1250.
Harvard	Shariati L et al. (2016) 'Comparison of Different Methods for Erythroid Differentiation in the K562 Cell Line', Biotechnology Letters, 38(8), pp. 1243-1250.
Vancouver	Shariati L, Modaress M, Khanahmad H, Hejazi Z, Tabatabaiefar MA, Salehi M, et al.. Comparison of Different Methods for Erythroid Differentiation in the K562 Cell Line. Biotechnology Letters. 2016;38(8):1243-1250.
BibTex	@article{ author = {Shariati L and Modaress M and Khanahmad H and Hejazi Z and Tabatabaiefar MA and Salehi M and Modarressi MH}, title = {Comparison of Different Methods for Erythroid Differentiation in the K562 Cell Line}, journal = {Biotechnology Letters}, volume = {38}, number = {8}, pages = {1243-1250}, year = {2016} }
RIS	TY - JOUR AU - Shariati L AU - Modaress M AU - Khanahmad H AU - Hejazi Z AU - Tabatabaiefar MA AU - Salehi M AU - Modarressi MH TI - Comparison of Different Methods for Erythroid Differentiation in the K562 Cell Line JO - Biotechnology Letters VL - 38 IS - 8 SP - 1243 EP - 1250 PY - 2016 ER -

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