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The Characteristics of an Ovine Lymphoid Cell-Line Sensitive to Vaccinal Infectious Bursal Disease Virus Strain Publisher Pubmed



Zandieh S1 ; Lotfi M2 ; Kamalzadeh M2 ; Shiri N2 ; Parmour E2 ; Eshaghi A2 ; Masoudi S3 ; Hablolvarid MH4 ; Shoushtari A5 ; Goudarzi H5 ; Taher Mofrad SMJ6 ; Amanpour S7
Authors
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Authors Affiliations
  1. 1. Department of Genetics, Faculty of Sciences, Islamic Azad University, Ahar, Iran
  2. 2. Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran
  3. 3. Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran
  4. 4. Department of Pathology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran
  5. 5. Management of Research and Diagnosis of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran
  6. 6. Department of Genetics, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
  7. 7. Center of Cancer Biology Research, Tehran University of Medical Sciences, Tehran, Iran

Source: Archives of Razi Institute Published:2017


Abstract

Infectious bursal disease (IBD), also known as Gumboro disease, is a globally well-known disease with a significant socio-economic effect. For control of IBD, several commercial egg- and cell-based vaccines are prepared. The cell-based IBD vaccines are significantly cost-effective; however, it is essential to confirm their safety and efficacy. The main cell line used to product the cell-based IBD vaccines, is a primary chicken embryo fibroblast (CEF). Nevertheless, manipulation of CEF is extremely challenging and time-consuming. This study aimed to characterize a sensitive suspension cell culture from ovine lymphoid, according to WHO technical report series; No. 978, Annex III. This authentication covered the growth curves, sensitivity, stability, karyotyping and identifying the adventitious agents. This cell line passed all defined tests and was considered as a suitable one for IBD vaccine preparation in a large scale. © Copyright 2017 by Razi Vaccine & Serum Research Institute.