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Effects of Repeated Exposure to Different Concentrations of Sevoflurane on the Neonatal Mouse Hippocampus; [Efeitos Da Exposicao Repetida a Diferentes Concentracoes De Sevoflurano Sobre O Hipocampo De Ratos Neonatos] Publisher Pubmed



Azimaraghi O1 ; Nezhad Sistani M2 ; Abdollahifar MA2 ; Movafegh A1 ; Maleki A3 ; Soltani E3 ; Shahbazkhani A4 ; Atefyekta R1
Authors
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Authors Affiliations
  1. 1. Tehran University of Medical Sciences, Dr. Shariati Hospital, Department of Anesthesiology and Critical Care Medicine, Teera, Iran
  2. 2. Shahid Beheshti University of Medical Sciences, Department of Anatomical Sciences, Teera, Iran
  3. 3. Tehran University of Medical Sciences, Children Medical Center Hospital, Department of Anesthesiology, Teera, Iran
  4. 4. Tehran University of Medical Sciences, Dr. Ali Shariati Hospital, Anesthesiology Research Development Center, Teera, Iran

Source: Brazilian Journal of Anesthesiology Published:2019


Abstract

Background and objectives: Developing brain is more vulnerable to environmental risk than is the developed brain. We evaluated the effects of repeated exposure to different concentrations of sevoflurane on the neonatal mouse hippocampus using stereological methods. Methods: Eighteen neonatal male mice were randomly divided into three groups. Group A, inhaled sevoflurane at a concentration of 1.5%; Group B, inhaled sevoflurane at a concentration of 3%; and Group C (control group), inhaled only 100% oxygen. Treatments were applied for 30 min a day for 7 consecutive days. The hippocampal volume, dendrite length, number of neurons, and number of glial cells were evaluated in each group using stereological estimations. Results: We identified a ∼2% reduction in the volume of the hippocampus in Group A compared to Group C. Mean hippocampal volume was ∼11% smaller in Group B than it was in Group C. However, these differences in hippocampal volume between the groups were not statistically significant (p > 0.05 for all). As for the number of neurons, we found significantly fewer neurons in Group A (∼29% less) and Group B (∼43% less) than we did in Group C (p < 0.05 for both). The dendrite length was ∼8% shorter in Group A and ∼11% shorter in Group B than it was in Group C. Conclusions: Repeated exposure to sevoflurane, regardless of the concentration, reduced the volume of the neonatal mouse hippocampus, as well as the number of neurons and dendrite length. © 2018 Sociedade Brasileira de Anestesiologia