Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells

Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells Publisher Pubmed

Tahamtan A^{1, 2} ; Tabarraei A¹ ; Moradi A¹ ; Dinarvand M³ ; Kelishadi M¹ ; Ghaemi A¹ ; Atyabi F⁴

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1. Department of Microbiology, Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
2. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
4. Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: Artificial Cells# Nanomedicine and Biotechnology Published:2015

Abstract

Chitosan nanoparticles (CS NPs) were prepared as a carrier for Human papillomavirus type 16 HPV-16) E7 gene and their gene transfection ability were evaluated in vitro . The plasmid expressing green fl uorescent protein (pEGFP) was used as a reporter gene. Gel electrophoresis demonstrated full binding of CS NPs with the pDNA. The transfection of CS-pEGFP NPs was effi cient in CHO cells and the expression of green fl uorescent proteins was well observed. The expression of E7 proteins was confi rmed under SDS-PAGE and western blot analysis. As a conclusion CS NPs may serve as an eff ective nonviral carrier for delivery of nucleotides into eukaryotic cells. Copyright © 2014 Informa Healthcare USA, Inc.

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Experts (# of related papers)

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Fatemeh Atyabi (4)

Rassoul Dinarvand (4)

Style	Citing Format
MLA	Tahamtan A, et al.. "Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells." Artificial Cells# Nanomedicine and Biotechnology, vol. 43, no. 6, 2015, pp. 366-372.
APA	Tahamtan A, Tabarraei A, Moradi A, Dinarvand M, Kelishadi M, Ghaemi A, Atyabi F (2015). Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells. Artificial Cells# Nanomedicine and Biotechnology, 43(6), 366-372.
Chicago	Tahamtan A, Tabarraei A, Moradi A, Dinarvand M, Kelishadi M, Ghaemi A, Atyabi F. "Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells." Artificial Cells# Nanomedicine and Biotechnology 43, no. 6 (2015): 366-372.
Harvard	Tahamtan A et al. (2015) 'Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells', Artificial Cells# Nanomedicine and Biotechnology, 43(6), pp. 366-372.
Vancouver	Tahamtan A, Tabarraei A, Moradi A, Dinarvand M, Kelishadi M, Ghaemi A, et al.. Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells. Artificial Cells# Nanomedicine and Biotechnology. 2015;43(6):366-372.
BibTex	@article{ author = {Tahamtan A and Tabarraei A and Moradi A and Dinarvand M and Kelishadi M and Ghaemi A and Atyabi F}, title = {Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells}, journal = {Artificial Cells# Nanomedicine and Biotechnology}, volume = {43}, number = {6}, pages = {366-372}, year = {2015} }
RIS	TY - JOUR AU - Tahamtan A AU - Tabarraei A AU - Moradi A AU - Dinarvand M AU - Kelishadi M AU - Ghaemi A AU - Atyabi F TI - Chitosan Nanoparticles As a Potential Nonviral Gene Delivery for Hpv-16 E7 Into Mammalian Cells JO - Artificial Cells# Nanomedicine and Biotechnology VL - 43 IS - 6 SP - 366 EP - 372 PY - 2015 ER -

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