Using Intron Splicing Trick for Preferential Gene Expression in Transduced Cells: An Approach for Suicide Gene Therapy

Using Intron Splicing Trick for Preferential Gene Expression in Transduced Cells: An Approach for Suicide Gene Therapy Publisher Pubmed

Pourzadegan F¹ ; Shariati L² ; Taghizadeh R¹ ; Khanahmad H^{1, 3} ; Mohammadi Z¹ ; Tabatabaiefar MA^{1, 3}

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1. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Hezarjarib Street, Isfahan, 8174673461, Iran
2. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Pediatric Inherited Diseases Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Cancer Gene Therapy Published:2016

Abstract

Suicide gene therapy is one of the most innovative approaches in which a potential toxic gene is delivered to the targeted cancer cell by different target delivery methods. We constructed a transfer vector to express green fluorescent protein (GFP) in transduced cells but not in packaging cells. We placed gfp under the control of the cytomegalovirus (CMV) promoter, which is positioned between the two long-terminal repeats in reverse direction. The intron-2 sequence of the human beta globin gene with two poly-A signals and several stop codons on the antisense strand was placed on the leading strand between the CMV promoter and gfp. For lentiviral production, the HEK293T and line were co-transfected with the PMD2G, psPAX2 and pLentiGFP-Ins2 plasmids. The HEK293T and line were transduced with this virus. PCR was performed for evaluation of intron splicing in transduced cells. The GFP expression was seen in 65% of the cells transduced. The PCR amplification of the genomic DNA of transduced cells confirmed the splicing of intron 2. The strategy is significant to accomplish our goal for preserving the packaging cells from the toxic gene expression during viral assembly and the resultant reduction in viral titration. Also it serves to address several other issues in the gene therapy. © 2016 Nature America, Inc. All rights reserved 0929-1903/16.

Style	Citing Format
MLA	Pourzadegan F, et al.. "Using Intron Splicing Trick for Preferential Gene Expression in Transduced Cells: An Approach for Suicide Gene Therapy." Cancer Gene Therapy, vol. 23, no. 1, 2016, pp. 7-12.
APA	Pourzadegan F, Shariati L, Taghizadeh R, Khanahmad H, Mohammadi Z, Tabatabaiefar MA (2016). Using Intron Splicing Trick for Preferential Gene Expression in Transduced Cells: An Approach for Suicide Gene Therapy. Cancer Gene Therapy, 23(1), 7-12.
Chicago	Pourzadegan F, Shariati L, Taghizadeh R, Khanahmad H, Mohammadi Z, Tabatabaiefar MA. "Using Intron Splicing Trick for Preferential Gene Expression in Transduced Cells: An Approach for Suicide Gene Therapy." Cancer Gene Therapy 23, no. 1 (2016): 7-12.
Harvard	Pourzadegan F et al. (2016) 'Using Intron Splicing Trick for Preferential Gene Expression in Transduced Cells: An Approach for Suicide Gene Therapy', Cancer Gene Therapy, 23(1), pp. 7-12.
Vancouver	Pourzadegan F, Shariati L, Taghizadeh R, Khanahmad H, Mohammadi Z, Tabatabaiefar MA. Using Intron Splicing Trick for Preferential Gene Expression in Transduced Cells: An Approach for Suicide Gene Therapy. Cancer Gene Therapy. 2016;23(1):7-12.
BibTex	@article{ author = {Pourzadegan F and Shariati L and Taghizadeh R and Khanahmad H and Mohammadi Z and Tabatabaiefar MA}, title = {Using Intron Splicing Trick for Preferential Gene Expression in Transduced Cells: An Approach for Suicide Gene Therapy}, journal = {Cancer Gene Therapy}, volume = {23}, number = {1}, pages = {7-12}, year = {2016} }
RIS	TY - JOUR AU - Pourzadegan F AU - Shariati L AU - Taghizadeh R AU - Khanahmad H AU - Mohammadi Z AU - Tabatabaiefar MA TI - Using Intron Splicing Trick for Preferential Gene Expression in Transduced Cells: An Approach for Suicide Gene Therapy JO - Cancer Gene Therapy VL - 23 IS - 1 SP - 7 EP - 12 PY - 2016 ER -

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