Ultrasensitive Quantitation of Flt3-Itd Mutation in Patients With Acute Myeloid Leukemia Using Ddpcr Publisher Pubmed



Kojabad AA¹ ; Chegeni R² ; Rostami S³ ; Zaker F¹ ; Safa M¹ Show Affiliations
Source: Molecular Biology Reports Published:2023

Abstract

Background: FLT3-ITD mutations occur in 45–50% of cytogenetically normal AML patients. Conventional fragment analysis using capillary electrophoresis is routinely used to quantitate FLT3-ITD mutations. Fragment analysis however has limited sensitivity. Methods and results: Here, FLT3-ITD was quantified in AML patients using an in-house developed ultra-sensitive droplet digital polymerase chain reaction assay (ddPCR). The allelic ratio of FLT3-ITD was also absolutely measured by both Fragment analysis and ddPCR. The sensitivity of ddPCR in quantitation of FLT3-ITD mutation was superior to Fragment analysis. Conclusion: This study demonstrates the feasibility of using the described in-house ddPCR method to quantify the FLT3-ITD mutation and measure FLT3-ITD AR in AML patients. © 2023, The Author(s), under exclusive licence to Springer Nature B.V.