The Inhibition of Epidermal Growth Factor Receptor Signaling by Hexagonal Selenium Nanoparticles Modified by Sirna

The Inhibition of Epidermal Growth Factor Receptor Signaling by Hexagonal Selenium Nanoparticles Modified by Sirna Publisher Pubmed

Kamrani Moghaddam L¹ ; Ramezani Paschepari S¹ ; Zaimy MA² ; Abdalaian A³ ; Jebali A⁴

Show Affiliations

1. Department of Developmental Cell Biology, School of Biological Sciences, Azad University, Tehran, 65526635, Iran
2. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Chemical Engineering, Princeton University, Princeton, NJ, United States
4. Advanced Medical Sciences and Technologies Department, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Source: Cancer Gene Therapy Published:2016

Abstract

The aim of this study was to investigate the effect of hexagonal selenium nanoparticles modified by SiRNA (HSNM-SiRNA) to inhibit epidermal growth factor receptor (EGFR) signaling in Human non-small-cell lung cancer (NSCLC). After synthesis, HSNM-SiRNA and HSNs were separately exposed to NSCLC cell lines (A549, H1299, H520, and H1975), and incubated for 6 h at 37 °C. Next, the expression of NFKB, MYC, STAT, ELK1, and GAPDH was evaluated by western blot and real-time PCR. The percentage of apoptotic cells and cell cycle progression were measured when exposed to HSNM-SiRNA and HSNs. Both western blot and real-time PCR results showed that HSNM-SiRNA could down-regulate the expression of all EGFR signaling genes. The percentage of apoptotic cells was significantly increased in all cell lines when exposed to HSNM-SiRNA (P>0.05). HSNM-SiRNA in A549 and H1299 cells significantly increased the proportion of cells in G1/G0 phase and significantly decreased the proportion of cells in S phase. © 2016 Nature America, Inc., part of Springer Nature.

Experts (# of related papers)

Mohammad Reza Nowroozi (1)

Abbas Shakoori farahani (1)

Style	Citing Format
MLA	Kamrani Moghaddam L, et al.. "The Inhibition of Epidermal Growth Factor Receptor Signaling by Hexagonal Selenium Nanoparticles Modified by Sirna." Cancer Gene Therapy, vol. 23, no. 9, 2016, pp. 321-325.
APA	Kamrani Moghaddam L, Ramezani Paschepari S, Zaimy MA, Abdalaian A, Jebali A (2016). The Inhibition of Epidermal Growth Factor Receptor Signaling by Hexagonal Selenium Nanoparticles Modified by Sirna. Cancer Gene Therapy, 23(9), 321-325.
Chicago	Kamrani Moghaddam L, Ramezani Paschepari S, Zaimy MA, Abdalaian A, Jebali A. "The Inhibition of Epidermal Growth Factor Receptor Signaling by Hexagonal Selenium Nanoparticles Modified by Sirna." Cancer Gene Therapy 23, no. 9 (2016): 321-325.
Harvard	Kamrani Moghaddam L et al. (2016) 'The Inhibition of Epidermal Growth Factor Receptor Signaling by Hexagonal Selenium Nanoparticles Modified by Sirna', Cancer Gene Therapy, 23(9), pp. 321-325.
Vancouver	Kamrani Moghaddam L, Ramezani Paschepari S, Zaimy MA, Abdalaian A, Jebali A. The Inhibition of Epidermal Growth Factor Receptor Signaling by Hexagonal Selenium Nanoparticles Modified by Sirna. Cancer Gene Therapy. 2016;23(9):321-325.
BibTex	@article{ author = {Kamrani Moghaddam L and Ramezani Paschepari S and Zaimy MA and Abdalaian A and Jebali A}, title = {The Inhibition of Epidermal Growth Factor Receptor Signaling by Hexagonal Selenium Nanoparticles Modified by Sirna}, journal = {Cancer Gene Therapy}, volume = {23}, number = {9}, pages = {321-325}, year = {2016} }
RIS	TY - JOUR AU - Kamrani Moghaddam L AU - Ramezani Paschepari S AU - Zaimy MA AU - Abdalaian A AU - Jebali A TI - The Inhibition of Epidermal Growth Factor Receptor Signaling by Hexagonal Selenium Nanoparticles Modified by Sirna JO - Cancer Gene Therapy VL - 23 IS - 9 SP - 321 EP - 325 PY - 2016 ER -

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