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A Novel Dna/Hemin Complex With Enzyme-Like Activity Selected From a Hairpin Dnas Library at Zero H2o2 Concentration Publisher



Shokri E1 ; Hosseini M2 ; Boldaji MN2 ; Shahsavar K2 ; Amirisadeghan A3 ; Nasiri N4 ; Bahmani A2 ; Ganjali MR5, 6 ; Saboury AA7
Authors
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Authors Affiliations
  1. 1. Department of Nanotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), P. O. Box: 31535-1897, Karaj, Iran
  2. 2. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, P. O. Box: 14395-1561, Tehran, Iran
  3. 3. Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, P. O. Box: 5166616471, Tabriz, Iran
  4. 4. Department of Biotechnology and Plant Breeding, Sari Agricultural Sciences and Natural Resources University (SANRU), P. O. Box: 578, Sari, Iran
  5. 5. Center of Excellence in Electrochemistry, School of Chemistry, College of Science, University of Tehran, P. O. Box: 14155-6455, Tehran, Iran
  6. 6. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, P. O. Box: 14155-6455, Tehran, Iran
  7. 7. Department of Biophysics, Institute of Biochemistry and Biophysics, University of Tehran, P. O. Box: 1365-13145, Tehran, Iran

Source: Molecular Catalysis Published:2022


Abstract

Here, a library of short DNAs possessing stem-loop conformation is examined for enzyme-like activity at zero H2O2 concentration. The stem part of all hairpins was identical and the loop sequences were diversified with runs of different nucleotides (A, T, C, G). According to the results, we found that G-type hairpins with 3, 4 and 5 guanines in the loop part are able to be complex with hemin and, oxidize TMB. Among them, a complex named G5HH showed an apparent oxidase-like activity and, was chosen for further studies. UV-thermal analysis, gel shift assay, mutation and structural tests with molecular docking results confirmed that G-tetrads are not formed in our experimental system. So, in this study we describe the functionality of a distinct type of DNA in the hairpin fold as a new class of DNAzymes. Discovering such property for simple DNA hairpins would hold great promise for wide applications in bio-nanotechnology and other fields of research. © 2022 Elsevier B.V.
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