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Up-Regulation of Sex-Determining Region Y-Box 9 (Sox9) in Growth Hormone-Secreting Pituitary Adenomas Publisher Pubmed



Shirian FI1 ; Ghorbani M2 ; Khamseh ME2 ; Imani M2 ; Panahi M3 ; Alimohammadi A4 ; Nourbakhsh M1 ; Salimi V5 ; Tavakoliyaraki M1
Authors
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Authors Affiliations
  1. 1. Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences (IUMS), Tehran, Iran
  3. 3. Firozgar Hospital, Pathology Department, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Iranian Legal Medicine Organizations, Tehran, Iran
  5. 5. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: BMC Endocrine Disorders Published:2021


Abstract

Background: Pituitary adenomas are benign brain tumors that cause considerable morbidity and neurological symptoms. SOX9 as a regulatory transcriptional mediator affects normal and tumor cell growth with an undefined role in pituitary adenomas pathogenesis. Thus, in the present study, the expression pattern of SOX9 in GH-secreting pituitary tumors and normal pituitary tissues is investigated. Methods: The SOX9 gene expression level was evaluated in 60 pituitary tissues including different types of GH-secreting adenomas and normal pituitary tissues through Real-Time PCR. The protein level of SOX9 was assessed using immunohistochemistry. The correlations of SOX9 gene and protein expression level with the patient’s clinical and pathological features were considered. Results: The SOX9 over-expression was detected in GH-secreting adenomas tumor tissues compared to normal pituitary tissues which were accompanied by overexpression of SOX9 protein in tumor tissues. The over-expression of SOX9 had a significant impact on GH-secreting adenomas tumor incidence with the odds ratio of 8.4 and the diagnostic value of SOX9 was considerable. The higher level of SOX9 expression was associated with invasive and macro tumors in GH-secreting pituitary adenoma patients. The positive correlation of SOX9 gene and protein level was observed and the tumor size and tumor invasive features were valuable in predicting SOX9 expression level in GH-producing pituitary tumors. Conclusion: The study provided the first shreds of evidence regarding the expression pattern of SOX9 in the GH- secreting pituitary adenomas at both gene and protein levels which may emphasize the possible involvement of SOX9 as a mediator in pituitary adenoma tumor formation also open up new intrinsic molecular mechanism regarding pituitary adenoma pathogenesis. © 2021, The Author(s).
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