Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Passive Immunity by Recombinant Anti-Methicillin-Resistant Staphylococcus Aureus Scaf Antibody in Mouse Model



Majelan PA1 ; Mahdavi M2 ; Pourmand MR3 ; Yazdi MH4 ; Khoramabadi N5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Medical Microbiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
  3. 3. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Research and Development, Pasteur Institute of Iran, Tehran, Iran
  5. 5. Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Source: Journal of Mazandaran University of Medical Sciences Published:2016

Abstract

Background and purpose: Methicillin resistant Staphylococcus aureus (MRSA) is a community-associated pathogen that is so common in hospitals. Antibiotic resistance and poor clinical outcome provide great reasons for using immunization strategies based on antibodies. The aim of this study was to investigate passive immunity using recombinant anti-MRSA ScaF antibody in a mouse model. Materials and methods: In this study, the recombinant protein, ScaF was purified through denaturation method. This protein combined with Freund’s adjuvant was injected into a rabbit to produce antibody. Then, the antibody produced against ScaF was injected to mice infected by S. aureus by two regimens of pre- and after infection treatment. Afterwards, the mortality rate in mice was compared between the control treatment group (vancomycin therapy) and negative control group (non-immune rabbit serum). Results: Out of 10 mice in two groups of pre- and after infection treatment that received antibody against ScaF antigen 20% and 10% were protected, respectively, that did not show a significant difference compared with non-immune rabbit serum group (negative control). Conclusion: Passive immunization by antibody cannot play a considerable role in the control of infections caused by S. aureus. Therefore, other mechanisms of immune system for the protection against this bacterium including stimulating of cytokine-dependent cell-mediated immunity may be useful in prevention of infections caused by S. aureus. © 2016, Mazandaran University of Medical Sciences. All Rights reserved.
Related Docs
1. Recombinant Staphylococcal Antigen-F (R-Scaf), a Novel Vaccine Candidate Against Methicillin Resistant Staphylococcus Aureus Infection: Potency and Efficacy Studies, Microbial Pathogenesis (2019)
2. Agammaglobulinemia: Epidemiology, Pathogenesis, Clinical Phenotype, Diagnosis, Prognosis and Management, Endocrine# Metabolic and Immune Disorders - Drug Targets (2020)
3. Protection of Mice Against Staphylococcus Aureus Infection by a Recombinant Protein Clfa–Isdb–Hlg As a Vaccine Candidate, Medical Microbiology and Immunology (2016)
4. X-Linked Agammaglobulinemia (Xla):Phenotype, Diagnosis, and Therapeutic Challenges Around the World, World Allergy Organization Journal (2019)
5. Evaluation of Respiratory Complications in Patients With X-Linked and Autosomal Recessive Agammaglobulinemia, Pediatric Allergy and Immunology (2020)
6. Agammaglobulinemia: Comorbidities and Long-Term Therapeutic Risks, Expert Opinion on Orphan Drugs (2017)
Experts (# of related papers)
View all Related Experts
Nima Rezaei (3)
Gholamreza Hassanpour (1)