Therapeutic Effects of Gallic Acid in Regulating Senescence and Diabetes; an in Vitro Study Publisher Pubmed



Rahimifard M¹ ; Baeeri M¹ ; Bahadar H² ; Moininodeh S¹ ; Khalid M¹ ; Haghiaminjan H³ ; Mohammadian H^{1, 4} ; Abdollahi M^{1, 4} Show Affiliations
Source: Molecules Published:2020

Abstract

Gallic acid (GA), a plant-derived ubiquitous secondary polyphenol metabolite, can be a useful dietary supplement. This in vitro study’s primary purpose was to assess the anti-aging properties of GA using rat embryonic fibroblast (REF) cells, antidiabetic effects via pancreatic islet cells, and finally, elucidating the molecular mechanisms of this natural compound. REF and islet cells were isolated from fetuses and pancreas of rats, respectively. Then, several senescence-associated molecular and biochemical parameters, along with antidiabetic markers, were investigated. GA caused a significant decrease in the β-galactosidase activity and reduced inflammatory cytokines and oxidative stress markers in REF cells. GA reduced the G0/G1 phase in senescent REF cells that led cells to G2/M. Besides, GA improved the function of the β cells. Flow cytometry and spectrophotometric analysis showed that it reduces apoptosis via inhibiting caspase-9 activity. Taken together, based on the present findings, this polyphenol metabolite at low doses regulates different pathways of senescence and diabetes through its antioxidative stress potential and modulation of mitochondrial complexes activities. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.