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Transforming Growth Factor Beta-Induced Factor 2-Linked X (Tgif2lx) Regulates Two Morphogenesis Genes, Nir1 and Nir2 in Human Colorectal Pubmed



Mobini GR1 ; Ghahremani MH2 ; Amanpour S3 ; Dehpour AR4, 5 ; Akbari A1, 6 ; Hoseiniharouni SM1 ; Muhammadnejad S3 ; Sheikhzade M7 ; Abedkhojasteh H8 ; Mohebi M8 ; Bolhassani M9 ; Heidari M5, 9
Authors
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Authors Affiliations
  1. 1. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Pharmacology and Toxicology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Cancer Models Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Experimental Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Colorectal Research Center, Rasoul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Biochemistry and Genetics, School of Medicine, Arak University of Medical Sciences, Arak, Iran
  8. 8. Farabi Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran
  9. 9. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Acta Medica Iranica Published:2016


Abstract

A member of homeodomain protein namely TGIF2LX has been implicated as a tumor suppressor gene in human malignancy as well as in spermatogenesis. However, to our knowledge, dynamic functional evidence of the TGIF2LX has not yet been provided. The aim of the present study was to investigate the human TGIF2LX target gene(s) using a cDNA-AFLP as a differential display method. A pEGFP-TGIF2LX construct containing the wild-type TGIF2LX cDNA was stably transfected into SW48 cells. UV microscopic analysis and Real-time RT-PCR were used to confirm TGIF2LX expression. The mRNA expressions of TGIF2LX in transfected SW48 cells, the cells containing empty vector (pEGFP-N), and untransfected cells were compared. Also, a Real-time PCR technique was applied to validate cDNA-AFLP results. The results revealed a significant down-regulation and up-regulationby TGIF2LX of Nir1 and Nir2 genes, respectively. The genes are engaged in the cell morphogenesis process. Our findings may provide new insight into the complex molecular pathways underlying colorectal cancer development. © 2016 Tehran University of Medical Sciences. All rights reserved.
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