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Quantum Dot-Labeled Tags Improve Minimal Detection Limit of Ca125 in Ovarian Cancer Cells and Tissues Publisher Pubmed



Shojaeian S1 ; Allameh A2 ; Jedditehrani M3 ; Ghods R4, 5 ; Shojaeian J6 ; Tabatabaeipanah AS7 ; Zarnani AH8, 9, 10
Authors
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Authors Affiliations
  1. 1. Department of Biochemistry, Medical Genetics, Nutrition, Alborz University of Medical Sciences, Karaj, Iran
  2. 2. Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
  3. 3. Department of Hybridoma, Monoclonal Antibody Research Center, Avicenna Research Institute, AcademicCenter for Education, Culture and Research (ACECR), Tehran, Iran
  4. 4. Oncopathology Research Center, Iran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medicine Sciences, Tehran, Iran
  6. 6. University of Maryland School of Pharmacy, Baltimore, MD, United States
  7. 7. Department of Biology, Faculty of Basic Sciences, Islamic Azad University, East Tehran Branch (Ghiamdasht), Tehran, Iran
  8. 8. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  9. 9. Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
  10. 10. Immunology Research Center (IRC), Iran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Allergy# Asthma and Immunology Published:2018


Abstract

In recent years, a lot of attention has been paid to quantum dot (QD) nanoparticles as fluorescent sensors for sensitive and accurate detection of cancer biomarkers. Here, using a homemade specific monoclonal antibody against CA125 and QD525- or FITC-labeled probes, expression of this marker in an ovarian cancer cell line and cancer tissues were traced and optical properties of fluorophores were compared qualitatively and quantitatively. Our results clearly showed that besides lower background and exceptionally higher photobleaching resistance, QD525 exhibited higher fluorescent intensity for both ovarian cancer cell and tissues at different exposure times (p<0.0001) and excitation filter sets (p<0.0001) exemplified by significantly higher staining index (p<0.016). More importantly, the FITC-labeled probe detected antigen-antibody complex at minimum concentration of 0.3 g/mL of anti-CA125, while reactivity limit decreased to 0.078 g/mL of anti-CA125 when QD525-labeled probe was applied showing four times higher reactivity level of QD525 probe compared to the same probe labeled with FITC. Based on our results, it seems that QDs are inimitable tags for sensitive detection and localization of ovarian cancer micrometastasis and molecular demarcation of cancer tissues in surgical practice, which subsequently figure out accurate therapeutic approaches. Copyright© August 2018, Iran J Allergy Asthma Immunol. All rights reserved.
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