Glutathione Responsive Chitosan-Thiolated Dextran Conjugated Mir-145 Nanoparticles Targeted With As1411 Aptamer for Cancer Treatment Publisher Pubmed



Tekie FSM^{1, 2} ; Soleimani M^{3, 4} ; Zakerian A³ ; Dinarvand M^{1, 5} ; Amini M⁶ ; Dinarvand R^{1, 2} ; Arefian E^{3, 7} ; Atyabi F^{1, 2} Show Affiliations
Source: Carbohydrate Polymers Published:2018

Abstract

miR-145 is a tumor suppressive miRNA which is abnormally reduced in different cancers. miR-145 overexpression reduces cancer migration, invasion, and cell adhesion. Increasing miR-145 level using suitable and efficient gene delivery systems could be valuable in cancer treatment. In this study, a redox-responsive miR-145 conjugated thiolated dextran (TD-miR) was prepared. Also, polyelectrolyte complexes (PECs) of TD-miR and chitosan were fabricated and decorated with anti nucleolin aptamer, AS1411 (apt-PEC). The size of the PECs was between 40–270 nm, and the zeta potential was varied according to the TD-miR to chitosan molar ratio. The outcomes of cellular studies indicated the excellence of the apt-PEC as a duel targeted delivery system and the PECs composed of chitosan 18 kDa with TD-miR to chitosan ratio of 5. TD-miR and the PECs are appropriate as the smart gene delivery systems which preserve and transfect the cargo and release it in cytoplasm. © 2018