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Comparing the Expression Levels of Gcg and Fbn-1 in the Plasma of Patients With Basal Cell Carcinoma (Bcc) and Healthy Individuals Publisher

Summary: Can biomarkers enhance skin cancer diagnosis? A study found that GCG and FBN1 are significantly elevated in basal cell carcinoma patients, suggesting their potential role in early detection. #SkinCancer #Biomarkers

Alamdari MK ; Mohamadnia A ; Bayat M ; Farhangiyan M ; Bahrami N
Authors

Source: Journal of Craniomaxillofacial Research Published:2025


Abstract

Introduction: One of the most common types of skin cancer is basal cell carcinoma (BCC), which puts a big burden on the healthcare system. Direct and dermoscopic examinations are used to diagnose basal cell carcinoma. GCG is a protein-coding gene expressed in various cells through-out the body, including the small intestine, brain, and skin. Fibrillin-1 is an extracellular protein found in many body tissues. In this study, we compare the expression of GCG and FBN1 genes in the blood of patients with BCC and a healthy control group. Materials and Methods: 1. Selection of patients and sampling. 2. Blood sampling of BCC patients and the control group. 3. Isolation of RNA from blood using an extraction kit. 4. Mea-surement of RNA concentration and purity. 5. cDNA synthesis and real-time PCR using a specific miRNA cDNA synthesis kit. 6. Statistical analysis Results: The GCG biomarker was positive in 9 out of 15 patients in the group of patients with basal cell carcinoma (BCC). The rate of positivity for this biomarker in the group of healthy individuals was 4 out of 15, indicating a statistically significant difference between the two groups (P-value<0.001). The FBN1 biomarker was positive in 11 out of 15 patients with basal cell carcinoma (BCC). The rate of positivity for this biomarker in the group of healthy individuals was 5 out of 15 people, indicating a statistically significant difference between the two studied groups. (P-value<0.001). Conclusion: The expression of GCG and FBN1 is significantly higher in patients with BCC compared to healthy individuals. Further studies can be done to ensure the role of these genes in the diagnosis of skin cancers. © 2025 Tehran University of Medical Sciences.