Integration and Evaluation of Cutaneous Leishmaniasis Laboratory Diagnosis in the Primary; [Integration Et Evaluation Du Diagnostic De La Leishmaniose Cutanee Dans Le Reseau De Laboratoires De Soins De Sante Primaires Resume] Publisher Pubmed



Zeinali M^{1, 2} ; Mohebali M^{1, 3} ; Shirzadi MR² ; Hassanpour G³ ; Behkar A³ ; Gouya MM² ; Samiee SM⁴ ; Malekafzali H⁵ Show Affiliations
Source: Eastern Mediterranean Health Journal Published:2023

Abstract

Background: The lack of an integrated national system prevents the Islamic Republic of Iran from registering and reporting all cases of cutaneous leishmaniasis. Aim: To establish a laboratory network for the improvement of diagnosis and surveillance of cutaneous leishmaniasis in endemic areas of the Islamic Republic of Iran using parasitological and molecular methods. Methods: This descriptive, cross-sectional, pilot study examined 49 laboratories in the 2 endemic areas for cutaneous leishmaniasis in the Islamic Republic of Iran. Samples were taken for identification of the dominant Leishmania species from individuals with cutaneous leishmaniasis referred to the laboratories and had not travelled to other endemic regions. Statistical analysis was conducted using SPSS version 25.0. Using the primary healthcare laboratory network, we established a 3-level surveillance system. We compared misdiagnosis, new cases, clinical relapses, treatment resistance, and treatment failure before and after establishment of the network. Results: Network implementation reduced relapse of cutaneous leishmaniasis. After the laboratory training, the average misdiagnosis rate decreased from 49.3% to 4.2% for positive microscopic slides and from 31.6% to 12% for negative slides. Correct diagnosis was significantly higher in the study areas after the intervention. Conclusion: Implementation of a cutaneous leishmaniasis laboratory network can enhance diagnosis, unify diagnostic methods and improve patient care. © Authors2023; Licensee: World Health Organization.