Development and Validation of a Short Runtime Method for Separation of Trace Amounts of 4-Aminophenol, Phenol, 3-Nitrosalicylic Acid and Mesalamine by Using Hplc System

Development and Validation of a Short Runtime Method for Separation of Trace Amounts of 4-Aminophenol, Phenol, 3-Nitrosalicylic Acid and Mesalamine by Using Hplc System Publisher

Siadati SA¹ ; Rezvanfar MA^{1, 2, 3} ; Payab M¹ ; Beheshti A^{1, 4}

Show Affiliations

1. R&D Department, Tofigh Daru Research and Engineering Company, TPICO, Tehran, Iran
2. Department of Toxicology and Pharmacology, Faculty of Pharmacy, and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences (TUMS), Tehran, Iran
3. Department of Toxicology and Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Chemistry, Payame Noor University, Tehran, Iran

Source: Current Chemistry Letters Published:2021

Abstract

In a short runtime process (about 6 minutes), with isocratic method without need of reconditioning before the next run, a mixture of some molecules containing 4-aminophenol (4APh), phenol, 3-nitrosalicylic acid (3NSA), and mesalamine (5-aminosalicylic acid) (MLZ), were separated and detected by means of HPLC/UV-Vis system. The resolutions of the separated and sharp peaks referring to the mentioned compounds were at least more than 2, and the tailing factor (TF) was about 1, even in 1 µg/l (ppm) concentration. In order to optimize the method, the effects of the pH, eluent percentage, flow rate, and buffer concentration, and wavelength on the chromatogram were investigated. Also, the results of the validation processes showed that the method trustable to be used in laboratories. © 2021 Growing Science Ltd.

Style	Citing Format
MLA	Siadati SA, et al.. "Development and Validation of a Short Runtime Method for Separation of Trace Amounts of 4-Aminophenol, Phenol, 3-Nitrosalicylic Acid and Mesalamine by Using Hplc System." Current Chemistry Letters, vol. 10, no. 3, 2021, pp. 151-160.
APA	Siadati SA, Rezvanfar MA, Payab M, Beheshti A (2021). Development and Validation of a Short Runtime Method for Separation of Trace Amounts of 4-Aminophenol, Phenol, 3-Nitrosalicylic Acid and Mesalamine by Using Hplc System. Current Chemistry Letters, 10(3), 151-160.
Chicago	Siadati SA, Rezvanfar MA, Payab M, Beheshti A. "Development and Validation of a Short Runtime Method for Separation of Trace Amounts of 4-Aminophenol, Phenol, 3-Nitrosalicylic Acid and Mesalamine by Using Hplc System." Current Chemistry Letters 10, no. 3 (2021): 151-160.
Harvard	Siadati SA et al. (2021) 'Development and Validation of a Short Runtime Method for Separation of Trace Amounts of 4-Aminophenol, Phenol, 3-Nitrosalicylic Acid and Mesalamine by Using Hplc System', Current Chemistry Letters, 10(3), pp. 151-160.
Vancouver	Siadati SA, Rezvanfar MA, Payab M, Beheshti A. Development and Validation of a Short Runtime Method for Separation of Trace Amounts of 4-Aminophenol, Phenol, 3-Nitrosalicylic Acid and Mesalamine by Using Hplc System. Current Chemistry Letters. 2021;10(3):151-160.
BibTex	@article{ author = {Siadati SA and Rezvanfar MA and Payab M and Beheshti A}, title = {Development and Validation of a Short Runtime Method for Separation of Trace Amounts of 4-Aminophenol, Phenol, 3-Nitrosalicylic Acid and Mesalamine by Using Hplc System}, journal = {Current Chemistry Letters}, volume = {10}, number = {3}, pages = {151-160}, year = {2021} }
RIS	TY - JOUR AU - Siadati SA AU - Rezvanfar MA AU - Payab M AU - Beheshti A TI - Development and Validation of a Short Runtime Method for Separation of Trace Amounts of 4-Aminophenol, Phenol, 3-Nitrosalicylic Acid and Mesalamine by Using Hplc System JO - Current Chemistry Letters VL - 10 IS - 3 SP - 151 EP - 160 PY - 2021 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract