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Antimicrobial Susceptibility, Serotyping, and Molecular Characterization of Antibiotic Resistance Genes in Listeria Monocyto-Genes Isolated From Pregnant Women With a History of Abortion Publisher



Heidarzadeh S1, 2 ; Pourmand MR1 ; Hasanvand S3 ; Pirjani R4 ; Afshar D2 ; Noori M4 ; Soltan Dallal MM1, 5
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Authors Affiliations
  1. 1. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
  3. 3. Department of Microbiology, Damghan Branch, Science and Research Islamic Azad University, Damghan, Iran
  4. 4. Department of Obstetrics & Gynecology, Arash Hospital, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Public Health Published:2021


Abstract

Background: Listeria monocytogenes show high mortality among pregnant women and newborns. This study aimed to detect L. monocytogenes in pregnant women with a history of abortion and assess the serotypes, antibiotic susceptibility patterns, and its resistance genes. Methods: Overall, 400 vaginal swabs were taken from pregnant women with a history of abortion in the past few years in a tertiary care hospital in Tehran, Iran, during 2015-2018. Antibiotics susceptibility to a panel of 10 antibiotics was determined using the standard disk diffusion method and the isolates serotyped by the aggluti-nation method. The antimicrobial-resistant isolates were also screened for the presence of tetM, ermB and dfrD genes by PCR. Results: Overall, 22 L. monocytogenes isolates were identified. High rates of resistance were observed for trime-thoprim (50%; n=11), sulphamethoxazole (50%; n=11), tetracycline (45.45%; n=10) and gentamicin (36.36%; n=8). From 22 L. monocytogenes isolates, 13 (59.10 %), 5 (22.73%), 3 (13.63%) and 1 (4.54%) belonged to sero-types 4b, 1/2a, 1/2b, and 3c, respectively. The genetic determinant tetM was detected in 70% of the tetracy-cline-resistant isolates. Out of 11 trimethoprim-resistant isolates, 27.27% isolates contained dfrD. Moreover, the ermB gene was found in 83.33% of the erythromycin-resistant isolates. Conclusion: Ampicillin and partly penicillin consider to be suitable antimicrobial agents to treat human listeri-osis. Moreover, due to resistance against many antibiotics, it is necessary to continue monitoring and managing antimicrobial resistance. © 2021, Iranian Journal of Public Health. All rights reserved.
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