Redox Responsive Functionalised Mesoporous Silica Nanoparticle for Targeted Drug Delivery of Doxorubicin Publisher Pubmed



Mojtabazadeh SS ; Samadi M ; Perseh S ; Saei S ; Bafkari R ; Atyabi F ; Dinarvand R
Source: Journal of Drug Targeting Published:2025

Abstract

Mesoporous silica nanoparticles (MSNs) have been studied for the delivery of anticancer drugs because of their unique mesoporous channels. In this study, Biotin was used as a targeting moiety of MSNs for the purpose of breast cancer cell targeting, and then, Gelatine grafting onto the surface of MSNs was carried out using glutaraldehyde-mediated cross-linking as a capping layer. Dynamic light scattering (DLS), Zeta potential change, infra-red spectroscopy (FT-IR), nitrogen adsorption and desorption (BET), and transmission electron microscopy (TEM) was used for the characterisation of size, morphology and other features related to the fabricated nanoparticles (NPs). The gelatine/biotin coated MSNs (MSN@Bio-Gel) were loaded with Doxorubicin (DOX), followed by assessing its drug loading and release behaviour. In vitro experiments were carried out for exploring the antitumor effect of DOX-MSN@Bio-Gel. The size of NPs prepared in this study was in the range of 178–286 nm. The MTT assay showed suitable anticancer activity of the NPs.Confocal microscopy showed that gelatine-coated, biotin-targeted MSNs had higher cell uptake into MCF-7 cancer cells. © 2025 Elsevier B.V., All rights reserved.