Targeting Human Telomerase Rna Component Using Antisense Oligonucleotide Induces Rapid Cell Death and Increases Ato-Induced Apoptosis in Apl Cells

Targeting Human Telomerase Rna Component Using Antisense Oligonucleotide Induces Rapid Cell Death and Increases Ato-Induced Apoptosis in Apl Cells Publisher Pubmed

Asgharikia L¹ ; Bashash D¹ ; Safaroghliazar A¹ ; Momeny M² ; Hamidpour M¹ ; Ghaffari SH²

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1. Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2. Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: European Journal of Pharmacology Published:2017

Abstract

The impressive advances carried out in designing pharmacological strategies with the aim of telomerase inhibition in cancers emerged a consensus that telomerase-targeted therapies could be exciting prospect in repertoire of future cancer strategies. The results of the present study indicated that targeting telomerase using an oligonucleotide-based molecule against human telomerase RNA template (hTR ASODN) reduced the survival rate of NB4 cells and induced a caspase-3-dependent apoptosis. Our finding was even noticeable in the synergistic experiments, where we found an enhanced reduction in the viability of the cells after short-term treatment with ATO in combination with the inhibitor. The resulting data delineated that short-term treatment of the cells with hTR ASODN either as single agent or in combination with ATO resulted in apoptotic cell death through activation of DNA damage response via up-regulation of p73 and ATM coupled with down-regulation of c-Myc. Moreover, we found that induction of p21 and subsequent disturbance of the death promoter to death repressor genes may contribute to the enhanced growth suppressive effect of the drugs combination. Overall, our findings support the idea that telomerase activity may have pivotal role in attenuating ATO effectiveness and combination of ATO with telomerase inhibitor seems to be a novel promising strategy, which may increase APL cure rates. © 2017 Elsevier B.V.

2. Contributory Role of Micrornas in Anti-Cancer Effects of Small Molecule Inhibitor of Telomerase (Bibr1532) on Acute Promyelocytic Leukemia Cell Line, European Journal of Pharmacology (2019)

3. Melatonin Promotes Ato-Induced Apoptosis in Mcf-7 Cells: Proposing Novel Therapeutic Potential for Breast Cancer, Biomedicine and Pharmacotherapy (2016)

4. Arsenic Trioxide and Bibr1532 Synergistically Inhibit Breast Cancer Cell Proliferation Through Attenuation of Nf-Κb Signaling Pathway, Life Sciences (2020)

5. Antileukemic Effects of Neurokinin-1 Receptor Inhibition on Hematologic Malignant Cells: A Novel Therapeutic Potential for Aprepitant, Anti-Cancer Drugs (2018)

6. Inhibition of Pi3k Pathway Using Bkm120 Intensified the Chemo-Sensitivity of Breast Cancer Cells to Arsenic Trioxide (Ato), International Journal of Biochemistry and Cell Biology (2019)

7. Enhanced Induction of Apoptosis in Chronic Myeloid Leukemia Cells Through Synergistic Effect of Telomerase Inhibitor Mst-312 and Imatinib, Molecular Biology Reports (2024)

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Style	Citing Format
MLA	Asgharikia L, et al.. "Targeting Human Telomerase Rna Component Using Antisense Oligonucleotide Induces Rapid Cell Death and Increases Ato-Induced Apoptosis in Apl Cells." European Journal of Pharmacology, vol. 809, no. , 2017, pp. 215-223.
APA	Asgharikia L, Bashash D, Safaroghliazar A, Momeny M, Hamidpour M, Ghaffari SH (2017). Targeting Human Telomerase Rna Component Using Antisense Oligonucleotide Induces Rapid Cell Death and Increases Ato-Induced Apoptosis in Apl Cells. European Journal of Pharmacology, 809(), 215-223.
Chicago	Asgharikia L, Bashash D, Safaroghliazar A, Momeny M, Hamidpour M, Ghaffari SH. "Targeting Human Telomerase Rna Component Using Antisense Oligonucleotide Induces Rapid Cell Death and Increases Ato-Induced Apoptosis in Apl Cells." European Journal of Pharmacology 809, no. (2017): 215-223.
Harvard	Asgharikia L et al. (2017) 'Targeting Human Telomerase Rna Component Using Antisense Oligonucleotide Induces Rapid Cell Death and Increases Ato-Induced Apoptosis in Apl Cells', European Journal of Pharmacology, 809(), pp. 215-223.
Vancouver	Asgharikia L, Bashash D, Safaroghliazar A, Momeny M, Hamidpour M, Ghaffari SH. Targeting Human Telomerase Rna Component Using Antisense Oligonucleotide Induces Rapid Cell Death and Increases Ato-Induced Apoptosis in Apl Cells. European Journal of Pharmacology. 2017;809():215-223.
BibTex	@article{ author = {Asgharikia L and Bashash D and Safaroghliazar A and Momeny M and Hamidpour M and Ghaffari SH}, title = {Targeting Human Telomerase Rna Component Using Antisense Oligonucleotide Induces Rapid Cell Death and Increases Ato-Induced Apoptosis in Apl Cells}, journal = {European Journal of Pharmacology}, volume = {809}, number = {}, pages = {215-223}, year = {2017} }
RIS	TY - JOUR AU - Asgharikia L AU - Bashash D AU - Safaroghliazar A AU - Momeny M AU - Hamidpour M AU - Ghaffari SH TI - Targeting Human Telomerase Rna Component Using Antisense Oligonucleotide Induces Rapid Cell Death and Increases Ato-Induced Apoptosis in Apl Cells JO - European Journal of Pharmacology VL - 809 IS - SP - 215 EP - 223 PY - 2017 ER -

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